活性污泥系统聚糖菌的富集与菌群多样性分析  

Enrichment and species analysis of glycogen accumulating organisms in activated sludge system

作　　者：李建民[1] 麻倩[1] 傅海霞[1] 董志英[1] 王凯丽[1] 

机构地区：[1]北京市环境保护科学研究院 国家城市环境污染控制工程技术研究中心 国家环境保护工业废水污染控制工程技术(北京)中心,北京100037

出　　处：《环境工程学报》2015年第3期1253-1258,共6页Chinese Journal of Environmental Engineering

基　　金：北京市环境保护科学研究院科技基金项目(2012-B-11)

摘　　要：在实验室规模续批式反应器(SBR)内,采用人工配水作为系统进水,以乙酸钠为唯一碳源,限制进水磷浓度,调整适宜的运行方式对活性污泥进行驯化,培养富集聚糖菌。90 d的培养过程中,系统的进水COD浓度始终维持在260mg/L左右,进水PO3-4-P浓度从20 mg/L逐渐降至2 mg/L,系统除磷能力逐渐丧失。系统内活性污泥的TP/TSS从40.5%降至10.4%,污泥中的糖原/TSS从14.5%增至38.2%,同时COD去除率能保持在90%以上,说明系统中成功富集聚糖菌。采用454高通量测序法分析该系统的活性污泥菌群结构,发现α-proteobacteria,β-proteobacteria,γ-proteobacteria 3类细菌占微生物总量的比例达83.78%。在属的等级上,Tepidicella占活性污泥菌群的比例最高,为20.60%。Glycogen accumulating organisms( GAOs) were enriched in a laboratory-scale sequencing batch reactor( SBR),in which acetate was the only organic substrate and the concentration of phosphate in the influent was controlled. During the 90 days' the experiment,the concentration of COD in the influent was maintained at260 mg / L and the concentration of PO3-4-P in the influent decreased from 20 mg / L to 2 mg / L,leading to a gradual decrease of phosphate removal efficiency. Throughout the experiment,the removal efficiency of COD was maintained above 90%,and the ratio between TP( total phosphate) and TSS( total suspended solid) of the activated sludge in the reactor decreased from 40. 5% to 10. 4% while the one between glycogen and TSS increased from 14. 5% to 38. 2%,which indicated that glycogen accumulating organisms were enriched successfully in the system. 454 high-throughput sequencing was applied to analyze the community of bacteria in the activated sludge within the reactor. The result revealed that α-proteobacteria, β-proteobacteria and γ-proteobacteria, up to83. 78%,were the dominant microbial in the sludge. And at genus level,Tepidicella was the richest,accounting for 20. 60%.

关 键 词：聚糖菌 菌群多样性 变形菌纲 454高通量测序 

分 类 号：X703[环境科学与工程—环境工程] X172