原子转移自由基聚合法亲水改性超大孔聚苯乙烯微球  

Hydrophilicity of Gigaporous Polystyrene Microspheres Prepared by Atom Transfer Radical Polymerization

作　　者：王少云[1] 张志刚[1] 张建平[1] 张荣月[2] 马光辉[2] 

机构地区：[1]河北科技师范学院化学工程学院,河北秦皇岛066004 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京100190

出　　处：《高分子材料科学与工程》2015年第6期181-184,共4页Polymer Materials Science & Engineering

基　　金：河北科技计划项目资助(13211221);国家自然科学基金资助项目(51103158;21336010)

摘　　要：采用原子转移自由基聚合(ATRP)方法,以超大孔聚苯乙烯微球表面的苄氯为引发位点,引发亲水性单体甲基丙烯酸羟乙酯(HEMA)在其表面进行接枝聚合,制备亲水性超大孔聚苯乙烯微球。改性后的微球的化学组成及表面形貌,分别通过傅里叶变换红外光谱和扫描电子显微镜进行了表征;亲水化的微球的接触角与改性前相比,从128.2°降到2.5°;同时测试了该微球对蛋白生物大分子的非特异性吸附,其蛋白回收率在90%以上,该亲水化的微球表面含有丰富的羟基,便于衍生为各种分离模式的生化分离介质,有望应用于蛋白快速分离纯化领域。A method for hydrophilicity of gigaporous polystyrene microspheres was explored through surface grafting polymerization of PHEMA initiated by atom transfer radical polymerization(ATRP).In this method,the groups of benzyl chlorides in the gigaporous polystyrene microspheres were used as initiator and 2-hydroxyethyl methacrylate(HEMA)was used for grafting monomer.Chemical composition and surface morphology of the modified microspheres were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy,respectively.The contact angle of the hydrophilic microspheres changes from 128.2°to 2.5°compared to the unmodified microspheres.Meanwhile,the protein recovery on the modified microspheres increases to be more than 90%.The hydrophilic microspheres with abundant hydroxyl are easy to be further derived as chromatographic supports for different separation modes.Its high permeability shows large potential in rapid separation and purification of proteins.

关 键 词：超大孔聚苯乙烯微球 原子转移自由基聚合 亲水改性 表面接枝 蛋白快速分离 

分 类 号：TQ325.2[化学工程—合成树脂塑料工业]