粤西地区两株鹅源鸭疫里氏杆菌的分离鉴定  

Isolation and identification of 2 strains of Riemerella anatipestifer from goose in the west of Guangdong Province

作　　者：彭凌[1,2] 杨旭夫[1,2] 朱必凤[1,2] 刘博婷 

机构地区：[1]韶关学院英东生命科学学院粤北生猪生产及疫病防控协同创新发展中心 [2]中国农业科学院哈尔滨兽医研究所-韶关学院动物疫病诊断中心联合实验室,广东韶关512005

出　　处：《中国兽医科学》2015年第5期463-468,共6页Chinese Veterinary Science

摘　　要：从疑似患有鸭疫里氏杆菌病的病死雏鹅分离病原菌并对分离到的菌株进行了形态学观察、生化试验、血清型鉴定、PCR鉴定、药敏试验及16SrRNA基因序列分析。结果显示,2个分离菌株为Ⅰ型鸭疫里氏杆菌;它们均对22种常用抗生素表现出耐药。这2株鹅源分离株与鸭源鸭疫里氏杆菌处于同一进化支上,与同属rRNA总科Ⅴ的伯杰氏菌属、金黄杆菌属、黄杆菌属的进化关系较近,与大肠杆菌、沙门菌、多杀性巴氏杆菌的进化关系较远。Pathogenic bacteria isolated from dead goslings suspected with Riemerella anatipestifer disease were identified by morphology,biochemistry tests,serological identification,PCR detection,drug sensitivity tests and sequence analysis of 16 SrRNA gene.In result,two isolates were proved to belong to the serotypeⅠ of R.anatipestifer.In the drug susceptibility tests,the two isolates were resistant to 22 kinds of antibiotic drug.Analysis of the 16 SrRNA gene sequences showed that the two isolates were at the same evolution branches with R.anatipestifer strains isolated from ducks.The phylogenetic tree confirmed that the phylogenetic position of the 2strains of R.anatipestifer have close evolutionary relationship with rRNA superfamilyⅤ of the genera Bergeyella,Chryseobacterium,and Flavobacterium,but have little relation to Escherichia coli,Salmonellaand Pasteurella multocida.

关 键 词：鹅 鸭疫里氏杆菌 16S RRNA基因 药敏试验 

分 类 号：S852.61[农业科学—基础兽医学]