葡萄风信子外植体直接再分化花芽和营养芽的研究  

Direct regenerated floral and vegetative buds from explants of Muscari armeniacum

作　　者：刘妮妮 刘雅莉[1,2] 娄倩 杨慧萍[1,2] 

机构地区：[1]西北农林科技大学旱区作物逆境生物学国家重点实验室/农业部西北地区园艺作物生物学与种质创制重点实验室,陕西杨凌712100 [2]西北农林科技大学林学院,陕西杨凌712100 [3]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2015年第6期187-192,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(31170652)

摘　　要：【目的】探索葡萄风信子直接再生花芽体系的建立,为葡萄风信子外植体直接再分化花芽研究提供参考。【方法】以葡萄风信子‘亚美尼亚’花蕾外植体为材料,MS为基础培养基,在均添加0.1mg/L 2,4-D的条件下,分别用不同质量浓度的6-BA(0,0.5,1,2,3,4mg/L)、GA3(0,0.5,1,2,3,4mg/L)和玉米素(0,0.05,0.1,0.5,1,2mg/L)处理,筛选出诱导花芽的最佳激素及最适质量浓度;以MS+6-BA(或玉米素)2mg/L+2,4-D 0.1mg/L研究开花时花瓣切片、花葶、叶片以及叶片诱导的愈伤组织诱导直接再分化花芽的情况。【结果】用MS+2mg/L 6-BA+0.1mg/L 2,4-D处理葡萄风信子花蕾时,可在花蕾基部直接再分化出具有蓝色的花芽,且花芽分化率最高,达60.2%。花葶、花瓣切片、叶片和愈伤组织在同样条件下只能诱导产生愈伤组织、营养芽。说明花蕾是诱导花芽分化的最佳外植体,且最适培养基为MS+2mg/L 6-BA+0.1mg/L 2,4-D。【结论】建立了葡萄风信子外植体直接再分化花芽体系。【Objective】The study investigated the optimal tissue culture conditions for Muscari armeniacumto directly induce the regeneration of floral buds.【Method】Floral buds explants of M.armeniacum were used as materials.With 0.1mg/L 2,4-D,the buds were treated with different concentrations of 6-BA(0,0.5,1,2,3,4mg/L),GA3(0,0.5,1,2,3,4mg/L)and ZT(0,0.05,0.1,0.5,1,2mg/L),respectively to select the optimal induction conditions for bud differentiation.Using MS+2 mg/L 6-BA(or ZT)+0.1mg/L 2,4-D,effects of different explants including young floral bud,petal segment,pedicel,young leaf and callus on the optimal induction conditions of flower bud were also studied.【Result】The optimal induction conditions for bud differentiation were MS+2 mg/L 6-BA+0.1 mg/L 2,4-D.The optimal induction explant was floral bud with induction rate of 60.2%.With the same hormones conditions,pedicel,sliced petals,leaf and callus explants can only be induced to form callus and vegetative buds.So the optimal induction explant is floral buds,optimal induction condition is MS+2mg/L 6-BA+0.1mg/L 2,4-D.【Conclusion】Optimal culture system for M.armeniacumto directly induce the bud differentiation was established.

关 键 词：葡萄风信子'亚美尼亚’ 直接再分化花芽 营养芽 组织培养 

分 类 号：S[农业科学]