桃砧木组织培养和扦插生根的解剖学观察  

Microscopic observation on adventitious root development of micropropagation and cutting propagation on peach rootstocks

作　　者：白晓燕[1] 王力荣[1] 王新卫[1] 朱更瑞[1] 方伟超[1] 曹珂[1] 陈昌文[1] 李勇[1] 

机构地区：[1]中国农业科学院郑州果树研究所,郑州450009

出　　处：《果树学报》2015年第1期74-78,175,共6页Journal of Fruit Science

基　　金：农业行业专项(201303093);国家作物种质资源保护和利用专项(2013NWB038);河南省科技创新人才计划

摘　　要：【目的】研究桃砧木茎尖快繁和扦插过程中不定根发生及发育解剖学特征。【方法】以桃砧木‘中桃抗砧1号’组培嫩梢和扦插枝条为试材,诱导其生根,通过常规石蜡切片法制片,对其生根部位进行解剖学观察。【结果】1)生根处理前,组培苗和插穗内均未发现潜伏根原基,生根处理后,2者均产生不定根原基。2)生根处理后,插穗内不定根原基在皮层、韧皮部、木质部中均可形成。3)不定根原基诱导后,有的不定根皮层结构中无中柱出现,有的分化出多个中柱,部分中柱与茎基部维管组织连通。【结论】‘中桃抗砧1号’的不定根原基为诱导型根原基,且属于多位点发生。【Objective】To investigate the anatomic characteristics and development of adventitious roots during tissue culture and cutting propagation in peach(Prunus persica L.)rootstocks.【Method】‘Zhong Tao Kang Zhen 1'shoots from tissue culture and cutting propagation were used as the experimental materials. The changes of morphology and anatomy were observed with paraffin method during adventitious root development after rooting treatments.【Results】1) There was no latent root primordium in tissue culture shoots and cuttings,but lots of adventitious root primordium developed after rooting treatments both in micropropagation and cutting propagation. 2) After rooting culture,the adventitious root primordium arose from the cortex,phloem,xylem and callus cells in cuttings. 3) Some adventitious roots had no stele in the cortex,the others had couple of steles and some steles connected with stem.【Conclusion】The adventitious root primordium of‘Zhong Tao Kang Zhen 1'was induce-to-root type and the development position of adventitious root belonged to multiple sites.

关 键 词：桃砧木 '中桃抗砧1号’ 愈伤组织 不定根根原基 解剖结构 

分 类 号：S662.1[农业科学—果树学]