Fusarium mangiferae对杧果顶芽内活性氧代谢的影响  

Effects of Fusarium mangiferae on active oxygen metabolism in apical bud of mango

作　　者：柳凤[1,2] 欧雄常[1] 詹儒林[1] 常金梅[1] 韦继光[2] 

机构地区：[1]中国热带农业科学院南亚热带作物研究所.农业部热带果树生物学重点实验室,广东湛江524091 [2]广西大学农学院,南宁530004

出　　处：《果树学报》2015年第1期94-99,共6页Journal of Fruit Science

基　　金：中央级非盈利性研究机构基本科研业务费研究专项(1630062014007);农业行业科研专项(201203092-3);四川省科技计划项目(2014NZ0026)

摘　　要：【目的】探讨杧果感染畸形病过程中顶芽内活性氧代谢的变化规律。【方法】以‘凯特’杧为试验材料,测定了接种病菌(Fusarium mangiferae)后顶芽内超氧阴离子(O2·)产生速率、过氧化氢(H2O2)、丙二醛(MDA)、抗坏血酸(As A)和谷胱甘肽(GSH)含量及活性氧清除相关酶(POD、CAT、SOD、APX和GR)活性的变化趋势。【结果】F.mangiferae侵染促进杧果顶芽O2·产生速率、H2O2和MDA含量的增加;抗氧化剂As A的含量在整个侵染过程中无明显变化,而GR含量急剧下降;在F.mangiferae侵染杧果后,POD、CAT、SOD和GR的活性迅速上升,且在整个测试过程中始终维持在较高水平,APX活性呈现先上升后下降的变化趋势。【结论】F.mangiferae侵染杧果后,导致大量活性氧爆发,细胞酶系统抗氧化能力下降,使其对活性氧的清除能力大大降低,加剧了活性氧的大量积累和对细胞的损伤。杧果活性氧代谢紊乱失衡可能是病菌的重要致病机理之一。【Objective】To study the effect of pathogens causing malformation on active oxygen metabolism in mango.【Method】We detected the changes of superoxide anion(O2·) production rate,hydrogen peroxide(H2O2),malondialdehyde(MDA),ascorbic acid(As A) and glutathiose(GSH) contents,and peroxidase(POD),catalase(CAT),superoxide dismutase(SOD),ascorbate peroxidase(APX) and glutathione reductase(GR) activities in mango infected by Fusarium mangiferae.【Result】The results showed that,the apical bud infected with F. mangiferae resulted in an increase of production rate,H2O2 and MDA contents. The content of As A had no obvious change,and the GR levels fell sharply. The activities of POD,CAT,SOD and GR increased rapidly after pathogen infection,and kept higher activities during the whole test period. The activity of APX increased at the early stage then decreased rapidly.【Conclusion】The pathogens could affect the defensive enzyme system in mango significantly,which leads to a large accumulation of reactive oxygen species,decrease in active oxygen scavenging capacity in the mango,and damages to cell finally. The antioxidant metabolism disturbances may be one of the important reasons for the disease.

关 键 词：杧果畸形病 侵染 活性氧 丙二醛 

分 类 号：S667.7[农业科学—果树学]