杏遗传多样性评价所需SSR等位变异数目估计  

An estimation of SSR alleles numbers for analyzing genetic diversity of apricot

作　　者：章秋平[1] 刘威生[1] 刘硕[1] 魏潇[1] 刘宁[1] 

机构地区：[1]辽宁省果树科学研究所,辽宁熊岳115009

出　　处：《果树学报》2015年第2期186-191,共6页Journal of Fruit Science

基　　金：国家自然科学基金项目(31401826和31071767);辽宁省农业领域青年科技创新人才培养计划(2014029)

摘　　要：【目的】研究等位变异数目的多少对杏材料间遗传关系分析结果的影响。【方法】以55对SSR引物对140份杏材料的检测结果为样本,运用矩阵相关检测的方法分析由不同SSR等位变异数目组成的相似矩阵与总等位变异相似系数矩阵之间的相关关系。【结果】所用SSR引物共检测到877个等位变异。随着抽取等位变异数目的增加,获得的相似矩阵与原矩阵(877等位变异)间的相关系数迅速增加。当选取的等位变异数目达到575时,与原等位变异共表型的相关性超过90%。【结论】所需最小等位变异数目与样本群体大小之间存在Y=149.5ln(X/12)+200.2的曲线方程,这个方程能够估算出在客观评价杏遗传群体时需要的最少SSR等位变异数目,并且认为这些SSR位点应尽量均匀分布在所有染色体上。【Objective】To analyze the impact of SSR alleles number on the genetic relationships in apri-cot.【Methods】All of the 55 SSR loci were used to test the genetic relationship among the 140 apricot ac-cessions. The similarity matrix of the general alleles(877) and those of different numbers of alleles from100 to 800 were compared by the methods of Mantel correlation test.【Results】We detected the 877 allel-ic variations in these SSR loci. The correlations coefficient between the sub-matrix and the general matrixdisplayed an upward tendency with increased numbers of alleles. There was good fit when the numbers ofalleles exceeded 575. The number of alleles required(Y) was significantly associated with the number ofsample(X) for good fit,and the regression equation was Y=149.5 ln(X/12) +200.2.【Conclusion】The trulygenetic relationship of apricot accessions could be obtained from enough alleles using SSR markers. Thisequation could be used to calculate the number of SSR alleles required for a genetic relationship study inapricot. The SSR primers might be selected according to well-proportioned distribution of the SSR loci inthe apricot genome.

关 键 词：杏 遗传多样性 微卫星 等位变异 矩阵相关检测 

分 类 号：S662.2[农业科学—果树学]