龙眼种质资源果实糖组分及含量特征分析  

Characteristics of soluble sugars in longan germplasm

作　　者：陈秀萍[1,2] 邓朝军[1,2] 胡文舜[1,2] 蒋际谋[1,2] 姜帆[1,2] 许奇志[1,2] 郑少泉[2,3] 

机构地区：[1]福建省农业科学院果树研究所,福州350013 [2]福建省龙眼枇杷育种工程技术研究中心,福州350013 [3]福建省农业科学院,福州350002

出　　处：《果树学报》2015年第3期420-426,共7页Journal of Fruit Science

基　　金：国家荔枝龙眼产业技术体系-龙眼育种岗位(CARS-33-05);农业部作物种质资源保护项目(2014NWB008);农业部热带作物种质资源保护项目(14RZZY-04);农业部农业科研杰出人才及其创新团队

摘　　要：【目的】对63份龙眼种质资源成熟果实中的可溶性糖组分及含量进行分析,了解龙眼种质资源果实糖分组成及含量特点。【方法】用高效液相色谱法测定,数据进行聚类分析。【结果】龙眼种质资源成熟果实中的可溶性糖主要包括蔗糖、果糖和葡萄糖,其中蔗糖含量最高,均值为94.14 mg·g-1,占总糖的62.5%;葡萄糖与果糖含量接近,分别占总糖的19.3%和18.2%;果糖、葡萄糖和蔗糖含量变异系数为25.0%~25.5%,蔗糖与单糖比值为0.75~4.83;总糖含量与甜度值、蔗糖与总糖含量间均呈极显著正相关(r=0.99**、0.82**),果糖与葡萄糖含量间呈极显著的直线正相关(斜率为1.060 5,R2=0.958 3);根据3种糖占总糖的比率,将63份龙眼种质资源分为2个类群5个组群,不同组群间果糖、葡萄糖和蔗糖含量占总糖的比率差异均达极显著水平。【结论】龙眼种质资源成熟果实中的可溶性糖主要由蔗糖、果糖和葡萄糖组成,均具有丰富的多样性,其中蔗糖含量最高,果糖和葡萄糖含量接近;63份龙眼种质资源分为糖组成特征明显的2个类群5个组群。【Objective】Components and contents of soluble sugars of 63 longan germplasm were deter-mined.【Methods】The high performance liquid chromatography(HPLC) was used to detect the compo-nents and contents of soluble sugars in ripening longan fruits,and the data were analyzed by cluster analy-sis.【Results】The results showed that the major components of soluble sugars in longan fruits were fruc-tose,glucose and sucrose. Sucrose had the highest content and with a mean of 94.14 mg·g-1,which ac-counted for 62.5% of the total sugar. Fructose and glucose contents were similar,which accounted for19.3% and 18.2% of the total sugar separately. The variation coefficient of the three sugars contents were25.0%~ 25.5%,and the value of the sucrose/mono were 0.75-4.83. The total sugar and sweetness value,sucrose and total sugar showed significantly positive correlation(r=0.99**,0.82**). Fructose and glucoseshowed significantly and linearly positive correlation,the slope is 1.060 5(R2=0.958 3). 63 longan germ-plasm could be classified into 2 groups and 5 subgroups,and these proportion showed extremely signifi-cant between different groups.【Conclusion】The major components of soluble sugars in ripening longan fruits were fructose,glucose and sucrose,which had abundant diversities. The sucrose content was thehighest one,fructose and glucose contents were similar. 63 longan germplasm could be classified into 2groups and 5 subgroups.

关 键 词：龙眼 种质资源 可溶性糖 高效液相色谱法 

分 类 号：S667.2[农业科学—果树学]