兔出血症病毒VP60蛋白与受体HBGAs结合位点的初步分析  

A preliminary analysis of binding points of rabbit haemorrhagic disease virus VP60 for histo-blood group antigens

作　　者：李珊珊[1,2,3] 范志宇[2] 胡波[2] 宋艳华[2] 魏后军[2] 彭伟[1,2] 李腾[1,2] 王芳[1,2] 恽时锋[1,3] 姜平[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]江苏省农业科学院兽医研究所农业部兽用生物制品工程技术重点实验室国家兽用生物制品工程技术研究中心,江苏南京210014 [3]南京军区南京总医院比较医学科,江苏南京210002

出　　处：《中国兽医科学》2015年第6期578-583,共6页Chinese Veterinary Science

基　　金：江苏省农业科技自主创新资金项目[CX(13)5029];江苏省自然科学基金资助项目(BK20140740)

摘　　要：通过血凝抑制试验筛选含H型组织血型抗原(HBGAs)的唾液样品,采用正交连续稀释法建立了兔出血症病毒(RHDV)衣壳蛋白VP60与HBGAs受体结合的酶联免疫方法,并用该方法检测了VP60蛋白的截短表达蛋白片段与HBGAs的结合情况,分析衣壳蛋白VP60与HBGAs的结合位点。结果表明,蛋白片段P250~350、P330~440、P330~350能与H型HBGAs结合,而蛋白片段P470~579、P559~579不能与H型HBGAs唾液结合。由此推断VP60蛋白与受体HBGAs结合位点大约在330~350aa区域内。本研究成功建立了HBGAs结合试验,为探索RHDV与宿主的相互作用提供了一种可靠的研究手段。To determine the structure-function relationship between capsid protein(VP60)of rabbit haemorrhagic disease virus(RHDV)and histo-blood group antigens(HBGAs),the HBGAs binding assay was established.The agglutination inhibition test was used to identify the types of HBGAs in H-type saliva sample,followed by detecting the binding of VP60 to H type HBGAs receptors.Meanwhile,the fragments of VP60 were detected,which showed that P250-350,P330-440 and P330-350 fragments of VP60 strongly binded to H-type saliva sample while P470-579 and P559-579 fragments could not bind to H-type saliva.In result,about 20 amino acids of the P330-350 fragment may be responsible for RHDV binding to HBGA receptors.The determination of binding domains may be helpful to characterize the molecular basis of the binding of RHDV to HBGAs and the infection mechanism of RHDV.The developed HBGAs binding assay provided a reliable method for exploration of the interaction between RHDV and hosts.

关 键 词：兔出血症病毒 VP60 组织血型抗原 HBGAs结合试验 

分 类 号：S852.65[农业科学—基础兽医学]