雏沙门菌S06004株毒力岛1缺失株的构建及其基本生物学特性的测定  

Construction and characterization of SPI1 deletion mutant of Salmonella pullorum S06004

作　　者：殷俊磊 程瞾 夏静 谢晓蕾 陶明新 李求春 耿士忠 焦新安 

机构地区：[1]扬州大学江苏省人兽共患病学重点实验室江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《中国兽医科学》2015年第6期639-643,共5页Chinese Veterinary Science

基　　金：国家自然科学基金重点项目(31230070);国家公益性行业(农业)科研专项(201403054);江苏省普通高校研究生科研创新计划项目(CXZZ13_0917);江苏省优势学科项目

摘　　要：为了解毒力岛1(SPI1)对雏沙门菌致病性的影响,初步探索研制安全有效的雏沙门菌减毒株,采用λ-red同源重组系统成功构建了雏沙门菌S06004株的SPI1(约40kb)缺失株S06004ΔSPI1。进一步的生物学特性研究显示,SPI1的缺失不影响雏沙门菌的生化特性和生长特性,且该缺失株具有良好的遗传稳定性,它对3日龄雏鸡的LD50是野生株的21倍。结果表明,SPI1的缺失则引起雏沙门菌毒力明显下降,这为进一步研究雏沙门菌SPI1的功能及制备减毒活疫苗奠定了一定的基础。In order to determine the effect of SPI1 on the pathogenicity of Salmonella pullorumand explore the feasibility of development of safe attenuated S.pullorumcandidate vaccine strains,the SPI1(40kb)deletion mutant of S.pullorumS06004 was constructed using theλ-red recombinant system and its biological characteristics were determined.The biochemical properties and growth property of S06004ΔSPI1were consistent with those of its parent strain S06004.The mutant was stable with the deletion of SPI1.The chick lethal test showed that the LD50 of S06004ΔSPI1was 21 times higher than that of the parent strain S06004 in 3-day-old chicks.In conclusion,the virulence of S06004ΔSPI1was obviously attenuated,and it provided basic data for further study of the SPI1 functions and for development of attenuated Salmonellavaccines.

关 键 词：雏沙门菌 λ-red同源重组 毒力岛1 生物学特性 

分 类 号：S852.612[农业科学—基础兽医学]