三氯乙烯致细胞免疫和体液免疫参与的混合型变态反应研究  

Involvement of cellular immunity and humoral immunity in mixed allergy induced by trichloroethylene

作　　者：徐新云[1] 李学余[1] 刘月峰[1] 

机构地区：[1]深圳市疾病预防控制中心毒理学研究室,518055

出　　处：《中华劳动卫生职业病杂志》2014年第12期881-886,共6页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：深圳市科技计划项目(200802073,201101015)

摘　　要：目的 探讨三氯乙烯(TCE)致变态反应是否存在细胞免疫和体液免疫共同参与,为研究其发病机制提供科学依据.方法 应用豚鼠和大鼠进行实验,分别设立阴性对照组、阳性对照组、TCE实验组,用皮内注射方式分别注射橄榄油、2,4-二硝基氯苯(DNCB)和TCE.实验结束后收集大鼠外周血液,用流式细胞仪检测淋巴细胞CD3+、CD4+、CD8+比例;收集豚鼠外周血液测定IgG、IgA、IgM、C3、C4水平;收集豚鼠脾淋巴细胞,用荧光定量PCR检测免疫相关基因GATA3、T-bet、CTLA4和Foxp3的mRNA表达水平.此外,选取TCE药疹样皮炎患者作为病例组,采用荧光定量PCR检测外周血Foxp3、GATA3、CTLA4、T-bet的mRNA表达水平.结果 (1)TCE对豚鼠皮肤有明显致敏作用,致敏率为83.3％;TCE实验组和阳性对照组IgG水平比阴性对照组显著升高(P＜0.01);TCE实验组和阳性对照组GATA3、T-bet、CTLA4 mRNA表达水平显著高于阴性对照组,Foxp3 mRNA表达水平低于阴性对照组.(2)TCE实验组和阳性对照组大鼠外周血淋巴细胞CD3+比例高于阴性对照组,TCE实验组CD4+、CD8+、CD4+/CD8+与阴性对照组比较无统计学差异.(3)TCE病例组Foxp3、GATA3、CTLA4 mRNA表达水平比对照组分别升高115％、97％和241％(P＜0.01),T-bet mRNA表达水平下降47％(P＜0.01).结论 TCE可引起细胞免疫和体液免疫发生明显改变,说明TCE导致的免疫损伤属于细胞免疫和体液免疫共同参与的混合型变态反应,可能是Ⅳ型和Ⅱ型变态反应.Objective To investigate whether cellular immunity and humoral immunity are involved in trichlorethylene (TCE)-induced mixed allergy,then provide the scientific basis for the mechanism of this disease.Methods Guinea pigs and rats were tested for this study by application of guinea pig maximization test (GPMT),the animals were randomly divided into negative control,positive control and TCE treatment groups.Animals of these groups were administrated with olive oil,2,4-dinitrochlorobenzene (DNCB),and TCE,respectively,by intradermal injection.After TCE administration,rat peripheral blood samples were collected by flow cytometry to detect lymphocytes CD3+,CD4+,CD8+.Guinea pig peripheral blood samples were collected to detect the levels of IgG,IgA,IgM,C3,C4,and the spleens were taken out from guinea pigs after various treatment,mRNA expression of GATA3,T-bet,CTLA4 and Foxp3 in lymphocytes of guinea pig spleen was detected by real-time fluorescent PCR assay.Additionally,TCE allergic dermatitis patients were selected for the study,the peripheral blood samples were collected from the TCE patients group and control group,quantitative PCR was applied to detect mRNA expression of immune-related genes Foxp3,GATA3,CTLA4,T-bet.Results TCE induced obvious skin allergic reaction in guinea pigs,the sensitization rate was 83.3％,IgG levels in TCE group and positive control increased significantly.Additionally,mRNA expression levels of GATA3,T-bet,CTLA4 significantly elevated in TCE group and positive control,but Foxp3 mRNA levels decreased.The lymphocytes CD3+ ratio in TCE group and positive control of rats was higher than that in negative control,we found that there was no statistical difference of CD4+,CD8+,CD4+/CD8+ between TCE group and negative control of rats.The mRNA expression levels of Foxp3,GATA3,CTLA4 in TCE patients increased by 115％,97％,241％,respectively as compared with the control,T-bet levels decreased by 47％ when compared with the control.Conclusions TCE could induce obvious changes of cellular immunity an

关 键 词：三氯乙烯 变态反应 细胞免疫 体液免疫 

分 类 号：R114[医药卫生—卫生毒理学]