人参皂苷Rg1对大鼠脾细胞功能的影响  

Effect of ginsenoside Rgl on the function of the spleen cells

作　　者：孙嘉政[1] 张晶[1] 邵月[1] 张力恒[1] 冉瑞图[1] 贾道勇 张梦思[1] 张岩岩[1] 王亚平[1] 

机构地区：[1]重庆医科大学干细胞与组织工程研究室,组织学与胚胎学教研室

出　　处：《解剖学杂志》2015年第4期392-392,449,共5页Chinese Journal of Anatomy

基　　金：国家自然科学基金(30973818);国家教育部博士导师基金(20125503110006);重庆医科大学学生创新基金(201314)

摘　　要：目的:探讨人参皂苷Rg1对大鼠脾结构与功能的影响及其初步机制.方法:Rg1注射组取脾,测定脾指数;石蜡切片H-E染色观察脾组织结构,计算脾白髓占脾组织切片的比例;衰老相关β-半乳糖苷酶(SA-β-Gal)染色检测脾细胞衰老情况;分离脾细胞,CCK-8检测其对刀豆蛋白A刺激的增殖能力;流式细胞术检测脾细胞增殖周期和细胞凋亡;ELISA检测脾细胞分泌肿瘤坏死因子-α(TNF-α)、粒细胞-巨噬细胞集落刺激因子(GM-CSF)、白细胞介素-2(IL-2)与白细胞介素-6(IL-6)的能力和晚期糖基化终产物(AGEs)、细胞活性氧(ROS)、丙二醛(MDA)、超氧化物歧化酶(SOD)含量,免疫印迹检测细胞衰老相关蛋白p53、p21、Rb的变化.结果:注射人参皂苷Rg1可使大鼠脾指数增加,脾白髓面积增加,脾细胞对刀豆蛋白A刺激的增殖能力提高,脾细胞S期比例提高,G1期及G2/M期比例相对下降,细胞凋亡减少,ROS、MDA水平降低,脾细胞分泌TNF-α、GM-CSF、IL-2与IL-6的能力增加,SOD活性提高,P21、P53、Rb蛋白表达下调.结论:人参皂苷Rg1具有保护脾结构和激活脾细胞功能的作用.Objective:To investigate the effect of ginsenoside Rgl on the splenic structure and function of rats and its preliminary mechanism.Methods:For group Rg1,the splenic indexes were measured,and paraffin sections were made to observe the microscopic structure of the spleen and calculate the proportion of the white pulp of the spleen.And senescenceaccociated β-galactosidase(SA-β-Gal) staining was applied to detect aging splenocytes.The proliferative capacity of splenocytes stimulated by concanavalin A(Con A) was measured by CCK-8.The cell cycles and apoptosis of splenocytes were assayed with flow cytometry.The amounts of tumor necrosis factor-α(TNF-α),granulocyte-macrophage colony stimulating factor(GM-CSF),interleukin-2(IL-2),interleukin-6(IL-6),advanced glycosylation end products(AGEs),reactive oxygen species(ROS),malondialdehyde(MDA),and superoxide dismutase(SOD) produced by splenocytes were assayed with ELISA.The expression of cell aging-related proteins p53,p21,Rb was detected by Western blotting.Results:The injection of ginsenoside Rgl could enhance the splenic index and the proportion of the white pulp of the spleen.The proliferative capacity of splenocytes and the ratio of the splenocyte in S stage were also improved.The secretory capability of TNF-α,GM-CSF,IL-2 and IL-6,and the active content of SOD increased in Rgl group while the ratio of the splenocyte in G1 and G2/M stage,the ratio of apoptosis and the production of ROS and MDA decreased.Ginsenoside Rgl could also downregulate the expression of p53,p21 and RB.Conclusion:Ginsenoside Rgl can protect the structure of the spleen and activate the function of splenocytes.

关 键 词：人参皂苷RG1 脾 结构 功能 大鼠 

分 类 号：R285.5[医药卫生—中药学]