Characterization of a novel toxin-antitoxin module,VapBC,encoded by Leptospira interrogans chromosome  

作　　者：YiXuanZHANG XiaoKuiGUO ChuanWU BoBI ShuangXiREN ChunFuWU GuoPingZHAO 

机构地区：[1]PharmaceuticalDepartment,ShenyangPharmaceuticalUniversity,103WenhuaRoad,ShenheDistrict,Shenyang110016,China [2]DepartmentofMicrobiologyandParasitology,ShanghaiSecondMedicalUniversity,280ChongqingnanRoad,Shangnai200025,China. [3]ResearchCenterofBiotechnology,ShanghaiInstitutesforBiologicalSciences,ChineseAcademyofSciences,500℃CaobaoRoad,Shanghai200233,China. [4]ChineseNationalHumanGenomeCenteratShanghai（CHGCS）,250BiboRoad,ZhangJiangHighTechPark,Shanghai,201203,China.

出　　处：《Cell Research》2004年第3期208-216,共9页细胞研究（英文版）

基　　金：This work was supported by the National High Technology Research and Development Program of China(Program No.2003AA223031).

摘　　要：Comparative genomic analysis of the coding sequences (CDSs) of Leptospira interrogans revealed a pair of closely linked genes homologous to the vapBC loci of many other bacteria with respect to both deduced amino acid sequences and operon organizations. Expression of single vapC gene in Escherichia coli resulted in inhibition of bacterial growth,whereas co-expression of vapBC restored the growth effectively. This phenotype is typical for three other characterized toxin-antitoxin systems of bacteria, i.e., mazEF[1], relBE[2] and chpIK[3]. The VapC proteins of bacteria and a thermophilic archeae, Solfolobus tokodaii, form a structurally distinguished group of toxin different from the other known toxins of bacteria. Phylogenetic analysis of both toxins and antitoxins of all categories indicated that although toxins were evolved from divergent sources and may or may not follow their speciation paths (as indicated by their 16s RNA sequences), co-evolution with their antitoxins was obvious.

关 键 词：Leptospria toxin-antitoxin system VapBC CO-EVOLUTION speciation. 

分 类 号：Q349[生物学—遗传学]