乙型肝炎病毒感染者HBV DNA与血清病毒标志物的关系  

Relationships among HBV DNA and HBV Immunological Markers in patients with chronic hepatitis type B

作　　者：周志红[1] 常曼丽[1] 张淑云[1] 刘伟[1] 杜博[1] 

机构地区：[1]哈尔滨医科大学第二临床医学院科研实验中心,黑龙江哈尔滨150086

出　　处：《哈尔滨医科大学学报》2004年第3期275-277,共3页Journal of Harbin Medical University

摘　　要：目的　探讨乙型肝炎病毒 (HBV)感染者HBVDNA检出情况及其与血清病毒标志物的关系。方法　采用实时荧光定量PCR(FQ PCR)检测 2 0 0份HBV感染者血清中HBVDNA含量 ,同时用全自动免疫分析仪检测HBV 5项血清标志物。结果　 2 0 0份血清中 ,乙型肝炎病毒标志物不同组合组的HBVDNA阳性率有差异 ,HBsAg、HBeAg和 (或 )HBcAb阳性组阳性率约为 98.1% ,其中 96 %以上HBVDNA≥ 10 4copy/ml;HBsAg、HBcAb和 (或 )HBeAb阳性组HBVDNA阳性率约为 76 .5 % ,但其中 90 %以上HBVDNA≤ 10 4copy/ml;单纯HBV抗体阳性组HBVDNA阳性率约为13.0 % ,但均为HBVDNA≤ 10 3 copy/ml。三组间HBVDNA阳性率及分布均有明显差异 (P <0 .0 5 )。而且HBVDNA含量与疾病严重程度相关。结论　FQObjective To investigate the relationship among HBV DNA and HBV Immunological Markers in patients with chronic hepatitis type B. Methods The amount of HBV DNA in the serum of 200 patients were estimated by FQ PCR.The serum HBV Immunological Markers of these patients were tested with ELISA .Results The amount of HBV DNA was different in 3 HBV Immunological Markers groups.The amount of HBV DNA in HBsAg,HBeAg and(or)HBcAb positive samples was higher than that in other groups with positive rate of 98.1%.The HBV DNA positive rate of HBsAg,HBcAb and(or) HBeAb positive samples was 76.5%; the positive rate of /HBcAb positive samples was 65%;the positive rate of HBcAb/HBeAb/HBsAb positive samples was 13%,but all of them ,HBV DNA≤10 3 copy/ml.Each group were difference in HBV DNA result.Conclusion FQ PCR has high sensitivity and specificity. The amount of HBV DNA with HBV Immunological Markers has important signification in diagnosis and judgement of patients with chronic hepatitis type B.

关 键 词：乙型肝炎病毒 HBV DNA 实时荧光定量PCR gI~清病毒标志物 

分 类 号：R512.62[医药卫生—内科学]