抗人DR5单克隆抗体诱导U343细胞凋亡研究  

Induction of Apoptosis in U343 Cell Line by a Novel Anti-Human DR5 Monoclonal Antibody

作　　者：庄国洪[1] 孙红光[1] 杜柏榕[1] 朱迅[1] 

机构地区：[1]吉林大学基础医学院免疫教研室,长春130021

出　　处：《中国肿瘤生物治疗杂志》2004年第2期96-99,共4页Chinese Journal of Cancer Biotherapy

基　　金：教育部<跨世纪优秀人才培养计划>资助项目

摘　　要：目的:探讨抗肿瘤坏死因子相关凋亡配体的死亡受体DR5单克隆抗体对神经胶质瘤细胞株U343的杀伤作用及机制。方法:采用流式细胞仪从蛋白质水平定量地检测DR5在U343的表达;用RT—PCR从mRNA水平检测DR5的表达;免疫细胞化学法明确DR5在U343的分布情况。通过MTT法、琼脂糖凝胶电泳、流式细胞仪DNA倍体分析,观察抗人DR5单克隆抗体对U343细胞增殖的抑制活性及其诱导凋亡效应。结果:神经胶质瘤细胞株U343表达死亡受体DR5,DR5分布于细胞内细胞核的周围。抗人DR5单克隆抗体3 μg/ml作用4 h可明显杀伤U343细胞,其机制与诱导凋亡有关。结论:抗人DR5单克隆抗体可以诱导神经胶质瘤细胞株U343凋亡,以死亡受体为靶点的抗体制剂为肿瘤治疗提供新的途径。Objective: To investigate the inhibitory effects and mechanism of a novel anti-human DR5 ( death receptor 5 of TRAIL) monoclonal antibody to glioma cell lines U343. Methods: DR5 protein was tested quantitative through FCM: DR5 mRNA was observed through RT-PCR and distribution was tested by immunocytochemistry. Inhibitory effects and inducing apoptosis of anti-human DR5 monoclonal antibody to U343 were analysed by MTT, DNA Ladder, FCM. Results: The expression of death receptor 5 ( DR5 ) was certificated in U343 , DR5 appeared to be located in intracellular perinucle-ar compartment. Inhibitory effects of anti-human DR5 monoclonal antibody on U343 were achieved by 3 μg/ml at 4 hours and the mechanism was associated with apoptosis. Conclusion: Apoptosis of glioma cell lines U343 can be induced by anti-human DR5 monoclonal antibody, and targeted on DR will provide new way to treating cancer.

关 键 词：肿瘤坏死因子相关凋亡诱导配体 死亡受体 凋亡 

分 类 号：R392.1[医药卫生—免疫学]