选择性去除骨髓移植物中异基因反应性淋巴细胞  

Selective Removal of Alloreactive Cells from Haematopoietic Stem Cell Grafts

作　　者：刘峰[1] 楼国良[1] 李永梅[1] 陈莉[1] 黄正霞[1] 杨宏斌[1] 朱杨[2] 王梁华[3] 球谊[3] 焦炳华[3] 

机构地区：[1]第二军医大学附属长海医院,上海200433 [2]第二医科大学附属新华医院,上海200433 [3]第二军医大学生化与分子生物学教研室,上海200433

出　　处：《中国肿瘤生物治疗杂志》2004年第2期110-113,共4页Chinese Journal of Cancer Biotherapy

基　　金：全军医学科研十五计划面上项目(01MA159)

摘　　要：目的:选择性去除骨髓移植物中异基因反应性淋巴细胞,诱导异基因特异性低反应性。方法:用携带有FasL基因的重组腺病毒转染BALB/c小鼠来源的DC,并与C57BL/6小鼠脾淋巴细胞共培养,通过混合淋巴细胞培养等方法检测异基因特异性低反应性。结果:转染FasL基因的DC诱导了对BALB/c异基因反应性T淋巴细胞凋亡,2次混合淋巴细胞反应显著降低,但并没有抑制针对第三方的反应。结论:转染FasL基因的DC体外可有效去除骨髓移植物中异基因反应性T淋巴细胞,移植用这种方法处理过的骨髓,有希望在抑制GVHD的同时,不影响移植物抗肿瘤复发和抗感染的功能。Objective: To obtain alloantigen-specific hyporesponsivness via depletion of alloreactive cells from haematopoietic stem cell grafts. Methods: BALB/c mouse bone marrow-derived dendritic cells genetically engineered to express FasL were cultured with C57BL/6 mouse spleen lymphocytes, and MLR was performed to evaluate the alloantigen-specific hyporesponsivness. Results: FasL-transfected DC induced T cell apoptosis and inhibited alloantigen-specific hyporespons-iveness in vitro. Conclusion: DC transfected with FasL gene may prevent graft-versus-host disease by selective removal of alloreactive cells from haematopoietic stem cell grafts

关 键 词：FASL 树突状细胞 移植物抗宿主病 骨髓移植 

分 类 号：R392.4[医药卫生—免疫学] R392.1[医药卫生—基础医学]