活化半相合混合骨髓移植GVHD反应的动物实验研究  

GVHD in Activated Half-Matched Mixed Bone Marrow Transplantation in Experimental Animal Models

作　　者：葛林阜[1] 姜国胜[2] 刘希民[1] 黄宁[1] 刘传芳[3] 唐天华[2] 董政军[1] 马焕文[1] 张玉昆[2] 孔凡盛[1] 郭鹏[1] 

机构地区：[1]济南军区总医院血液科,济南250031 [2]山东省医学科学院基础所血液肿瘤学研究室,济南2500622 [3]山东大学齐鲁医院血液肿瘤中心,济南250012

出　　处：《中国肿瘤生物治疗杂志》2004年第2期129-132,共4页Chinese Journal of Cancer Biotherapy

基　　金：济南军区"十五"医药卫生重点课题02275

摘　　要：目的:观察活化半相合混合骨髓移植的GVHD反应。方法:以急性放射病615小鼠模型为受鼠,615×C57BL/6杂交F1代小鼠为半相合供鼠。半相合鼠骨髓和脾细胞中混合一定比例的同基因脾细胞,经白细胞介素-2体外活化后,进行活化半相合混合骨髓移植。观察移植后小鼠死亡率、白细胞系造血重建、脾结节、体内混合淋巴细胞培养、嵌合体及病理学改变,比较不同移植方式的GVHD反应。结果:活化半相合骨髓移植组与未活化半相合骨髓移植组具有明显的GVHD反应。供受鼠3:1活化半相合混合骨髓移植不能明显降低GVHD反应,供受鼠脾细胞比例1:1和2:1移植组可以明显减轻GVHD反应。表现为外周血自细胞及骨髓造血恢复快,体内混合淋巴细胞反应降低及嵌合体百分率升高。结论:活化半相合混合骨髓移植方式可以降低GVHD反应,并与同基因和异基因脾细胞比例有关。Objective: To observe graft-versus-host disease (GVHD) reaction in activated half-matched mixed bone marrow transplantation (BMT) . Methods; Mice 615 with acute radiation disease were used as recipient mice model, with 615C57BL/6 hybrid F1 mice being half-matched donor mice. Half-matched murine bone marrow and spleen cells mixed with certain percent of syngeneic spleen cells were activated in vitro with IL-2 and underwent mixed half-matched BMT to observe their death rate, hematopoietic recovery of myelocytic lineage, spleen node count, mixed lymphocyte reaction in vivo, chimerism and pathological changes, to compared with GVHD reactions of various transplant models. Results: GVHD was relatively severe in both activated and non-actived half-matched BMT groups, showing that activated half-matched mixed 3: 1 BMT group after activation could not alleviate GVHD, whereas the activated half-matched mixed 1:1 BMT group or 2: 1 group with high percentage of syngeneic spleen cells could lessen the extent of GVHD, as manifested by the quick recovery of peripheral blood WBC count and bone marrow GM-CFU formation, by the low of mixed lymphocyte reaction in vivo as well as by the increase of chimeric rate. Conclusions: Half-matched mixed BMT after activation could lower the GVHD reaction, which was relative to the ratio of syngeneic to allogeneic spleen cells.

关 键 词：骨髓移植 半相合 移植物抗宿主病 小鼠 

分 类 号：R457.7[医药卫生—治疗学] R392.4[医药卫生—临床医学]