2型糖尿病合并冠心病患者纤溶酶原激动抑制剂-1基因多态性分析  

4G/5G Polymorphism at Plasminogen Activator Inhibitor-1 Promoter in Patients with Type Ⅱ Diabetic and Coronary Artery Disease

作　　者：王旭东[1] 樊峥[2] 傅研[1] 殷红[2] 翟艳玲[2] 祁雅慧[2] 朱宇清[1] 王瑾[1] 赵相印[3] 

机构地区：[1]首都医科大学附属北京同仁医院急诊科 [2]首都医科大学实验中心 [3]北京同仁医院血液科

出　　处：《首都医科大学学报》2004年第2期223-226,共4页Journal of Capital Medical University

摘　　要：为探讨纤溶酶原激活抑制剂 1 (PAI 1 )基因调节区 -675bp位点 4G/5G多态性与 2型糖尿病、2型糖尿病合并冠心病 (CAD)的关系 ,采用特异性引物聚合酶链反应 (PCR)方法 ,测定 4G/5G基因型 ,并检测PAI 1抗原水平 ,共观察了 1 2 1例单纯 2型糖尿病 (typeⅡdiabetes)患者、87例 2型糖尿病合并CAD患者和 1 0 0例对照者。结果 :合并CAD组的 4G/4G基因型携带者明显高于对照组 ( 4 9.4%比 3 7.0 % ,P <0 .0 5 ) ,CAD组中PAI 1与三酰甘油 (TG)呈正相关 ,并与 4G/5G多态性有关 ,表现为 4G/4G基因型携带者的PAI 1质量浓度明显高于 5G/5G基因型携带者〔( 3 9.82× 1 0 - 3± 3 .1 1× 1 0 - 3)ng/L比 ( 3 8.0 4× 1 0 - 3± 2 .73× 1 0 - 3)ng/L ,P <0 .0 5〕。提示 :PAI 1基因 4G/5G多态性与TG的联合作用与PAI 1抗原水平相关 ,并与 2型糖尿病合并CAD的发病过程有关。The aim was to investigate the genotype at a plasminogen activator inhibitor-1 (PAI-1) promoter polymorphism in patients with type Ⅱ diabetes and complicated coronary artery disease(CAD). The level of PAI-1 antigen was measured and the 4G/5G polymorphism genotype was determined by polymerase chain reaction(PCR) amplification of genomic DNA using the allele specific primers in Chinese type Ⅱ diabetes subjects(121 type Ⅱ diabetes patients, 87 diabetic patients with CAD) and 100 control subjects. The frequency of the 4G/4G genotype increased and that of the 5G/5G genotype decreased in group of diabetic CAD compared with control subjects(49.4% vs. 37.0%, P< 0.05 ). The level of PAI-1 antigen was positively correlated with levels of triglyceride(P<0.001) and associated with 4G/5G polymorphism (P=0.03). PAI-1 antigen level was significantly higher in 4G/4G genotype carriers than in 5G/5G genotype carriers in group of CAD 〔(39.82×10 -3 ±3.11×10 -3 )ng/L vs ( 38.04× 10 -3 ±2.73×10 -3 )ng/L, P<0.05〕. This polymorphic variation at PAI-1 promoter interacts with risk factors of triglyceride when associated with increased level of PAI-1 antigen and pathogenesis of CAD in type Ⅱ diabetes.

关 键 词：2型糖尿病 冠心病 纤溶酶原激动抑制剂-1 基因多态性 

分 类 号：R587.1[医药卫生—内分泌]