精子密度与来源对卵胞浆内单精子注射结果影响的研究  

The impact of the density and originations of sperm on the therapentic outcome of ICSI

作　　者：刘新宇[1] 文江[1] 刘娟[1] 

机构地区：[1]沈阳204医院,110041

出　　处：《辽宁医学杂志》2004年第1期4-6,共3页Medical Journal of Liaoning

摘　　要：目的　自 1999年 1月至 2 0 0 2年 4月行卵胞浆内单精子注射 (ICSl)治疗 30 2个周期 ,研究精子密度及精子来源对卵胞浆内单精注射 (ICSI)治疗结果的影响。方法　采用GnRH -A +FSH/HMG +HCG(促性腺激素释放激素 +绝经期尿促性腺激素 +绒毛膜促性腺激素 )方案超促排卵 ,对MⅡ卵行显微注射 ,培养箱内培养 2～ 3天 ,将优质胚胎 2～ 3枚回植宫腔 ,观察受精率、卵裂率及妊娠率。结果　 (1)体外排精 +ICSI、PESA(附睾取精 ) +ICSI、TESA(睾丸取精 ) +ICSI三组的受精率分别为 70 .6 %、6 7.5 %和 5 8.8% ,其 χ2 =15 .5 6 ,P <0 .0 5 ;卵裂率分别为 94 0 %、94 4 %和 93 0 % ,P >0 .0 5 ;妊娠率分别为 32 .0 %、36 .7%和 2 4 .3% ,P >0 .0 5。 (2 )精子密度在≤ 1× 10 4/ml组、>1× 10 4/ml～ <1× 10 6/ml组、≥ 1× 10 6/ml组的受精率分别为 6 1.6 %、70 .4 %和 71.8% ,其 χ2 =2 3.0 6 ,P <0 .0 5 ;卵裂率为 94 .3%、93.5 %和 92 .4 % ,P >0 .0 5 ;妊娠率分别为 32 .4 %、34.1%和 2 3 8% ,P >0 .0 5。结论　 (1)TESA +ICSI组的受精率明显低于另两组 ,卵裂率、优质胚胎率与妊娠率无显著差异 ;(2 )精子密度极低(≤ 1× 10 4/ml)时受精率显著下降。Objective Retrospective summrize the 225 cycles outcomes of ICSI in our ART center and analyze the different factors impact including sperm count,sperm origin,female age on the outcome of ICSI.Methods Microinject sperm into the M Ⅱ oocyte retrieved by COH. Culture the fertilized oocyte for 2~3days and transplant 2~3 embryos of high quality into the uterus. Results 302 treatment cycles obtained 1938 stage M Ⅱmaturated oocytes,the rate of fertilization was 70.6%,of cleavage was 94%,clinical pregnant rate 32%.Conclusions (1)Fertilization rate in TESA group is statistically lower than other two groups,while no statistically difference in cleavage rate and pregnancy rate;(2)Fertilization rate is statistically lower in poor semen ( ≤ 1 ×10 4/ml). introcytoplasmic sperm injection percutaneous epididymal sperm aspiration(PESA) testicular sperm extraction(TESA) sperm density sperm origination

关 键 词：精子密度 来源 卵胞浆内单精子注射 睾丸切开取精 

分 类 号：R711.6[医药卫生—妇产科学]