人绒毛膜促性腺激素(HCG)的生物电化学免疫测定  

BIOELECTROCHEMICAL IMMUNOASSAY FOR HUMANCHORIONIC GONADOTROPHIN

作　　者：吕鸣祥[1] 申群英[1] 张杰[1] 

机构地区：[1]天津大学

出　　处：《中国生物医学工程学报》1991年第4期203-207,共5页Chinese Journal of Biomedical Engineering

基　　金：中科院长春应用化学所电分析开放研究室资助课题

摘　　要：本文介绍了在玻璃碳电极上共价结合HCG抗体的免疫电极。采用循环伏安法测定了免疫电极的循环伏安图谱。当电极上抗体与抗原发生反应,形成抗原-抗体络合物后,循环伏安曲线的阴极峰电流发生变化。根据阴极峰电流的变化,可以测定溶液中HCG的含量,测定的浓度范围为5.0×10^(-8)～5.0×10^(-6)克/毫升。<ABSTRACT> In this paper a cyclic voltammetry for an anti-HCG antibody coval- ently attached to a glassy cardon electrode is described.When the antigen- antibody complex is formed on the surface of the immunoelectrode, the cathodic peak current varied. According to the variation, the amount of HCG in the solution is determined. The sensitivity of the assay is 5.0 × 10-8g/ml, being higher then non-labeled immunosensor.

关 键 词：人绒毛膜促性腺激素 HCG 生物电化学免疫测定 免疫电极 循环伏安图谱 

分 类 号：R446.6[医药卫生—诊断学]