蛇毒纤溶酶的分离纯化及其溶栓作用  

Purification of fibrinoenase from snake venom and its fibrinolytic effect

作　　者：马骉[1] 张骞[2] 宋梦薇 吴丹[1] 林琳[3] 付学奇[2] 林永齐[2] 

机构地区：[1]北京赛生药业有限公司,北京100176 [2]吉林大学生命科学学院,吉林长春130023 [3]大连医科大学生化室,辽宁大连116027

出　　处：《中国生化药物杂志》2004年第3期156-158,180,共4页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的获得大量高纯度的蛇毒纤溶酶并进行药效实验。方法用离子交换柱色谱和单克隆抗体亲和色谱技术进行纯化 ,用体外和半体内血栓模型研究其抗血栓作用。结果得到纯度为 95 %以上的纤溶酶。PurposeThe aim is to establish a new method for purification of the fibrinoenase from snake venom by affinity chromatography.MethodsMonoclonal antibody affinity chromatograpy was used. ResultsThe purity of fibrinoenase of above 95% by HPLC was obtained.ConclusionThe purified method was efficient for purifying fibrinoenase.The results indicate that the fibrinoenase had valuable fibrinolytic effect.

关 键 词：蛇毒 纤溶酶 溶栓 亲和色谱 

分 类 号：Q556[生物学—生物化学] R972[医药卫生—药品]