BSA-ELISA法检测隐孢子感染者mIL-2R水平的逻辑质研究  

Logic qualitative study on changes of mIL-2R in patients infected by cryptosporidium parvum with BSA-ELISA

作　　者：叶松[1] 徐有魁[1] 孙善才[1] 王克霞[1] 许礼发[1] 

机构地区：[1]安徽理工大学医学院病原学与免疫学教研室,淮南232001

出　　处：《中国卫生检验杂志》2004年第3期274-275,共2页Chinese Journal of Health Laboratory Technology

基　　金：安徽理工大学科技基金资助项目(项目号 :2 0 0 0 - 73)

摘　　要：〔目的〕由逻辑质方法探讨隐孢子虫病患者的免疫细胞膜白介素 -2受体 (mIL -2R)表达功能的特征。〔方法〕采用生物素 -链霉亲和素 (Biotin -Streptavidin ,BSA)法对卵囊阳性患者分别进行外周血T细胞亚群、膜白介素 -2受体 (mIL -2R)检测 ;进行逻辑质指标分析。〔结果〕在静息期和诱导期 ,隐孢子虫卵囊阳性者的免疫细胞膜白介素 -2受体表达水平与卵囊阴性者mIL -2R相比 ,在逻辑上存在显著差异 (t≤ 0 93 0 7,P >0 0 5 ;t≥ 4 63 88,P <0 0 5 )。〔结论〕隐孢子虫感染者免疫细胞的mIL -2R表达水平降低。〔Objective〕 To investigate the changes of expression of immune cell mIL-2R in patients infected by cryptosporidium parvum using the method of logic qualitative determination.〔Methods〕 BSA-ELISA was used to detect T subsets and membrane mIL-2R in peripheral blood mononuclear cells (PBMC) of the patients.Meanwhile,the index analysis of logic qualitative research was performed.〔Results〕 The cell positive rate of immune cell mIL-2R expression in the patients was significantly different from that in healthy individuals in the cellular immune rest period and inducing period.〔Conclusions〕 The expression level of immune cell mIL-2R is decreased in the patients infected by cryptosporidium parvum.

关 键 词：BSA-ELISA法 隐孢子感染 MIL-2R 逻辑质 免疫细胞 

分 类 号：R446.6[医药卫生—诊断学]