大隐静脉曲张血管内光凝治疗后LPO,SOD,GSH-Px的变化  

Changes of Blood Levels of LPO, SOD and GSH-Px after Endovenous Laser Treatment of Varicose Greater Saphenous Vein

作　　者：韩丽娜[1] 顾瑛[1] 刘凡光[1] 

机构地区：[1]中国人民解放军总医院激光科,100853

出　　处：《放射免疫学杂志》2004年第3期193-195,共3页Journal of Radioimmanology

摘　　要：目的 :比较血管内激光光凝治疗大隐静脉曲张后和传统静脉高位结扎 ,分段剥脱手术治疗大隐静脉曲张后血液中过氧化脂质 (LPO)、超氧化物歧化酶 (SOD)和谷胱甘肽过氧化物酶 (GSH -Px)的表达。方法 :70例大隐静脉曲张患者 ,37例行血管内激光光凝治疗 ,33例行传统大隐静脉高位结扎 ,分段剥脱手术。分别在术前、术后第 1,3,5 ,7,10 ,15天取静脉血 ,测定LPO、SOD和GSH -Px的水平 ,测定方法分别为硫代巴比妥酸法(TBA)荧光法 ,放射免疫分析法和二硫双硝基苯甲酸 (DTNB)直接法。另外取 30例健康成人作为对照。结果 :大隐静脉曲张患者LPO水平较正常水平高 ,SOD、GSH -Px水平较正常水平低。两种术式治疗后LPO水平均升高 ,随着病情的恢复逐渐下降 ,接近正常水平 ;SOD和GSH -Px先下降后升高 ,升高的时间落后于LPO。血管内光凝组患者LPO的增高水平低于静脉结扎、剥脱治疗组 ,而且恢复至正常水平的时间先于静脉结扎、剥脱组。结论 :LPO、SOD和GSH -Px的表达水平与手术创伤和术后病情恢复情况密切相关。Objective To investigate the changes of the blood levels of lipid peroxide (LPO)、 superoxide dimutase (SOD) and glutathione peroxidase (GSH-Px) after treatment of varicose greater saphehous vein with either endovenous laser or conventional surgery (high ligation plus stripping). Methods Thirty-seven patients with varicose greater saphenous vein were treated with endovenous laser and another 33 patients were treated with conventional surgery. Levels of LPO (serum, with TBA fluorescin)、 SOD (whole blood, with RIA) and GSH-Px (whole blood, with direct DTNB) were determined in these patients both before and 1, 3, 5, 7, 10, 15 days after treatment. Levels in 30 controls were also measured. Results The levels of LPO were higher and levels of SOD、 GSH-Px lower in the patients than those in the controls. After either form of therapy, the levels LPO rose and levels of SOD、 GSH-Px dropped immediately but gradualy approached the control values by the 15 th day (slower with SOD and GSH-Px). However, the early increase of LPO levels were less and recovery sooner in the group of patients treated with laser. Conclusion Changes of levels of LPO、 SOD and GSH-Px were closely related to the degree of stress and recovery condition after the treatment.

关 键 词：大隐静脉曲张 血管内光凝 LPO SOD GSH-PX 过氧化脂质 超氧化物歧化酶 

分 类 号：R543.6[医药卫生—心血管疾病]