RP-HPLC Determination of Pinoresinol Diglucopyranoside in Qing′e Pill Extract  

RP-HPLC法测定青娥丸提取物中松脂醇二葡萄糖苷含量(英文)

作　　者：熊志立[1] 罗璇[1] 李小芩[1] 李发美[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《Journal of Chinese Pharmaceutical Sciences》2004年第2期138-141,共4页中国药学（英文版）

摘　　要：Aim To develop and determine pinoresinol diglucopyranoside in Qing'e Pill, atraditional Chinese compound preparation containing Eucommia ulmoides Oliv. as the principal drug,by a reverse-phase high-performance liquid chromatographic method (RP-HPLC) . Methods The extract ofQing'e Pill was refluxed with 75% ethanol, purified on an AB-8 macroporous adsorption resin columnand then injected into HPLC system. The HPLC assay was performed on an ODS analytical column with amixture of methanol-acetonitrile-water (24:3:78, V/V/V) as the mobile phase at a flow-rate of 1.0mL·min^(-1), and a UV detector set at 227 nm. Results Good linearity between peak area andconcentration was found in the range of 5.5 - 170 μg·mL^(-1) for pinoresinol diglucopyranoside ( r> 0.9998) . The average recovery was 99.3%. The intra-day assay RSD and the inter-day assay RSDwere 1.3% and 2.8%, respectively (n = 5). The content of pinoresinol diglucopyranoside in Qing'ePill was determined to be 0.446 +- 0.012 mg·g^(-1) (n = 10). Conclusion The RP-HPLC method wasproved to be sensitive, specific, accurate and precise for the determination of pinoresinoldiglucopyranoside in Qing' e Pill.目的　建立测定青娥丸提取物中松脂醇二葡萄糖苷含量的RP HPLC法。方法　青娥丸提取物用 75 %乙醇提取 ,经AB 8大孔吸附树脂纯化后 ,注入高效液相色谱仪。本法采用ODS分析柱为固定相 ,以甲醇 乙腈 水 (2 4∶3∶78,V V V)为流动相 ,流速为 1 0mL·min- 1 ,紫外检测波长为 2 2 7nm。结果　松脂醇二葡萄糖苷的线性范围为 5 5 170 μg·mL- 1 (r>0 9998) ,平均回收率 99 3%。日内和日间精密度分别为 1 3%和 2 8% (n =5 )。青娥丸中松脂醇二葡萄糖苷的含量为 0 4 4 6± 0 0 12mg·g- 1 (n =10 )。结论　本方法灵敏、准确 ,专属性强 。

关 键 词：RP-HPLC pinoresinol diglucopyranoside qing'e pill 

分 类 号：O657.7[理学—分析化学]