抗rSj14-3-3单克隆抗体检测循环抗原及疗效考核价值的初步研究  

Evaluation of anti-rSj14-3-3 monoclonal antibody on detection of circulating schistosoma antigen following chemotheraphy of praziquantel

作　　者：查任远[1] 沈继龙[1] 汪学龙[1] 

机构地区：[1]安徽医科大学病原生物学教研室,合肥230032

出　　处：《安徽医科大学学报》2004年第3期204-207,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金资助 (编号 :3 0 170 841);安徽省自然科学基金资助 (编号 :0 0 44 5 47)

摘　　要：目的　探讨抗重组日本血吸虫 14 3 3(rSj14 3 3)的单克隆抗体检测血吸虫循环抗原对血吸虫病疗效考核的价值。方法　制备纯化的抗日本血吸虫 14 3 3的单克隆抗体 ,以纯化的兔抗rSj14 3 3多抗和酶标羊抗兔多抗为检测系统的酶联免疫吸附试验 (P M ELISA)法 ,分别对同批治疗前及治疗后 2、4、6、12个月的血吸虫病患者和感染兔血清进行检测 ,并与常规检测抗体的可溶性虫卵抗原 -酶联免疫吸附试验 (SEA ELISA)法比较。结果　P M ELISA法对治疗前血吸虫病患者检测阳性率为 98 2 % ,而检测抗体的SEA ELISA为 95 5 %。P M ELISA法对治愈后 2、4、6、12个月血清循环抗原检测阴转率分别为 17 7%、4 1 9%、5 5 %、85 % ,而检测抗体的SEA ELISA法分别为 3 2 %、6 5 %、12 5 %、15 %。结论　该法既有较好的诊断价值 ,又有较好的疗效考核价值。Objective To explore the value of anti-rSj14-3-3 monoclonal antibody on detection of circulating antigen following the treatment of praziquantel in schistosomiasis.Methods P-M-ELISA was established with purified anti-rSj14-3-3 monoclonal antibody applied for the capture antibody,and purified anti-rSj14-3-3 polyclonal antibody plus goat anti-rabbit antibody IgG linked with HRP applied for the detecting antibody.P-M-ELISA was applied for detection of circulating antigen in serum samples of patients with schistosomiasis before and 2, 4, 6, 12 months after treatment,and compared with SEA-ELISA for detection of antibodies. Results The positive rate was 98.2% by using P-M-ELISA for detecting circulating antigen in patients with schistosomiasis before treatment,while the positive rate of anti-SEA antibody was 95.5%.The reversion rate of patients at 2, 4, 6, 12 months after treatment were 17.7%?41.9%?55%?85% respectively,but with SEA-ELISA were 3.2%, 6.5%, 12.5%, 15%, respectively. Conclusion P-M-ELISA with anti-rSj14-3-3 monoclonal antibody has high value on diagnosis and evaluation of chemotheraphy efficacy of schistosomiasis.

关 键 词：血吸虫病 日本/免疫学 抗原 抗体 单克隆 治疗 结果 重组 SJ14-3-3 

分 类 号：R532.21[医药卫生—内科学] R392.11[医药卫生—临床医学]