梨S系矮化砧组培苗增殖系数和质量的影响因子  

Influencing Factors on Multiplication Coefficient and Regeneration Quality of Adventitious Shoots of the S Series Dwarfing Rootstock of Pear

作　　者：及华[1] 张海新[2] 赵玉芬 葛会波[4] 

机构地区：[1]河北省农林科学院遗传生理研究所,河北石家庄050051 [2]河北林业学校,河北石家庄050061 [3]河北省林业科学院,河北石家庄050061 [4]河北农业大学园艺学院,河北保定071000

出　　处：《河北农业科学》2004年第2期40-44,共5页Journal of Hebei Agricultural Sciences

基　　金：河北省科技攻关项目 ( 972 45 5 0 6D)

摘　　要：对梨S系矮化砧试管苗增殖系数和生长状态的影响因子进行了研究 ,结果表明 :S1 ～S6 不同砧木类型试管分化能力存在差异 ;培养基中添加 1 0mg/L的 6 BA ,既能保证试管苗较高的增殖率 ,又能保证试管苗的质量 ;试管苗再生能力随继代培养代数的增加而提高 ;培养基中GA3≤ 0 1mg/L和使用组培专用封口膜 ,可以有效提高试管苗的分化质量 ,防止玻璃苗产生。The influencing factors of multiplication coefficient and regeneration quality for adventitious shoots of the S series dwarfing rootstock of pear were studied. The results showed: The regeneration capability of the adventitious shoots in different rootstocks was different. The multiplication coefficient and the quality of the shoots were higher on culture media with 1.0 mg/L 6-BA. The more the subculture times were,the higher the regeneration capability was.keeping GA 3 concentration under 0.1 mg/L and use of appropriative sealing material could improve the quality of adventitious shoots and overcome vitrification.

关 键 词：梨 S系矮化砧 组培苗 增殖系数 分化质量 继代培养 

分 类 号：S661.2[农业科学—果树学]