丹参酮ⅡA对大鼠局灶性脑缺血再灌注损伤的保护作用  

Protective Effect of Tanshinone ⅡA Against Focal Cerebral Ischemia Reperfusion Injury in Rats

作　　者：叶龙彬[1] 奚涛[2] 陈峰[3] 王瑜[1] 

机构地区：[1]南京大学生命科学院,南京210093 [2]中国药科大学生物技术研究中心,南京210009 [3]中国药科大学分析测试中心,南京210009

出　　处：《中国药科大学学报》2004年第3期267-270,共4页Journal of China Pharmaceutical University

摘　　要：目的 :观察丹参酮ⅡA对大鼠局灶性脑缺血再灌注损伤的保护作用。方法 :采用线栓法制备大鼠局灶性脑缺血再灌注模型 ,以水溶性的丹参酮ⅡA纳米制剂灌胃给药 ,观测其对脑缺血大鼠的神经行为、脑梗塞范围和脑含水量以及脑组织SOD活力、MDA含量和NO水平的影响。结果 :丹参酮ⅡA(2 5mg/kg)能使脑缺血大鼠的神经行为明显改善 ,脑梗塞范围和脑含水量显著降低 ;丹参酮ⅡA亦能拮抗脑缺血再灌注引起的SOD活力的下降及MDA含量的升高 ,并使脑组织NO水平显著下降。结论 :丹参酮ⅡA对大鼠局灶性脑缺血再灌注损伤具有很好的保护作用。AIM:To investigate the protective effect of TanshinoneⅡA against brain injury in rats undergone focal ischemia reprefusion.METHOD:The model of rat focal cerebral ischemia was made through midlle cerebral artery occlusion-reperfusion and irrigated preparation of nanometer TanshinoneⅡA.The behavior,infarct size and water content of ischemic rats were evaluated.The SOD activity,MDA and NO content were measured.RESULT: TanshinoneⅡA could significantly improve the behavior of ischemic rats and markedly decrease their infarct size and water content.The drug could also enhance the SOD activity and reduce the MDA and NO content in rats' brain tissue at the same time.CONCLUSION:TanshinoneⅡA has protective effect against focal brain damage induced by occlusion-reperfusion in middle cerebral artery of rats.

关 键 词：丹参酮ⅡA 纳米制剂 局灶性脑缺血 自由基 一氧化氮 

分 类 号：R285[医药卫生—中药学]