西红花苷大鼠小肠吸收研究及油水分配系数的测定  

Studies on The Intestinal Absorption of Crocin in Rats and Determination of the Partition Coefficient

作　　者：金玉燕[1] 张钧寿[1] 张瑛[2] 张玉红[1] 

机构地区：[1]中国药科大学药剂教研室,南京210009 [2]东南大学化学化工系,南京210096

出　　处：《中国药科大学学报》2004年第3期283-284,共2页Journal of China Pharmaceutical University

摘　　要：AIM:To clarify both the character of the absorption and the stability of crocin in various sites along the intestinal tract,and also to determine the partition coefficient of crocin-1 in water/n-caprylic alcohol system. METHOD:The intestinal absorption and stability was investigated using in situ perfusion method in rats; Shaking flask test was used to determine the partition coefficient of crocin-1;Concentration of crocin-1 was detected by HPLC.RESULT:Decrease of Crocin-1 in various intestine segments is as follows:Duodenum 13.81%,Jejunum 9.89%,Ileum 10.07%,and Colon 10.04%,but crocin-1 can’t be found in blood; Degradation of Crocin-1 during incubation in refluxing solution of various intestinal segments is as follows:Duodenum 13.01%,Jejunum 10.11%,Ileum 9.95%,and Colon 10.45%; The partition coefficient of crocin-1 P =0.093±0.004,log P =-1.03±0.02.CONCLUSION:The results suggest that crocin is unstable and can’t be absorbed in the whole intestinal tract.AIM:To clarify both the character of the absorption and the stability of crocin in various sites along the intestinal tract,and also to determine the partition coefficient of crocin-1 in water/n-caprylic alcohol system. METHOD:The intestinal absorption and stability was investigated using in situ perfusion method in rats; Shaking flask test was used to determine the partition coefficient of crocin-1;Concentration of crocin-1 was detected by HPLC.RESULT:Decrease of Crocin-1 in various intestine segments is as follows:Duodenum 13.81%,Jejunum 9.89%,Ileum 10.07%,and Colon 10.04%,but crocin-1 can't be found in blood; Degradation of Crocin-1 during incubation in refluxing solution of various intestinal segments is as follows:Duodenum 13.01%,Jejunum 10.11%,Ileum 9.95%,and Colon 10.45%; The partition coefficient of crocin-1 P =0.093±0.004,log P =-1.03±0.02.CONCLUSION:The results suggest that crocin is unstable and can't be absorbed in the whole intestinal tract.

关 键 词：西红花苷 小肠吸收 稳定性 油水分配系数 

分 类 号：R285[医药卫生—中药学]