广州市儿童A族链球菌T型连续7年调查  

An epidemiological study of group A hemolytic streptococci isolated from elementary school children in Guangzhou since 1988 to 1994

作　　者：董太明[1] 吴红穗[1] 陈剑光[1] 陈志红[1] 苏健[1] 黄震东[1] 

机构地区：[1]广东省人民医院心内科,广州市510100

出　　处：《岭南心血管病杂志》2004年第3期165-170,共6页South China Journal of Cardiovascular Diseases

摘　　要：目的　调查广州市学龄儿童咽部携带A族链球菌 (GAS)T型在不同时期的流行规律和特点。方法　从1988年 9月至 1994年 7月 ,分别在广州市越秀区 (城市 ) ,广州市番禺区大石镇 (农村 )采用随机整群抽样的方法在小学3～ 5年级的小学生中抽取 2～ 3个班学生作为研究对象 ,研究时间分别为 1988年 9月至 1989年 8月、1990年 5月至1991年 4月、1992年 9月至 1993年 7月、1993年 8月至1994年 7月共 4个学年度 ,跨度达 7年的长期观察和研究 ;研究期内每个月对每个受试者进行一次咽拭子培养 ,将分离出的 β 溶血性链球菌进行杆菌肽试验 ,兰菲分组 ,T分型和OF分型。结果　共分离出 2 5 30株GAS ,T型分布结果依序为T不可分型 (UT)占 17 6 8%、T6 11 2 3%、T4 9 2 9%、T17 79%、T115 6 1%、T8/ 2 5 /IMP195 4 2 %和T5 / 2 7/ 4 44 98%等 7个占总菌株 5 %以上的主导菌型 ;城乡两地菌型差异较大 (P <0 0 0 1) ;城乡两地T型在不同年份变化较大(P <0 0 0 1) ;4 2 4株GAS作OF试验 ,结果OF阴性菌株占6 5 8%。结论　广州市 1988年 9月至 1994年 7月之间 ,学龄儿童咽部流行的GAST型主导菌株依序为T6、4、1、11、8/2 5 /IMPl9和 5 / 2 5 / 4 4为主 ,城乡两地流行的主导菌型差异较大 。Objectives To investigate the epidemiological characteristic of the T typing of group A streptococci (GAS) isolated from elementary school children in Guangzhou from 1988 to 1994. Methods During a 7-year period between 1988 and 1994, total 1 687 elementary school children aged 9～14 years old were selected randomly as subjects in Guangzhou city, 953 children from Guangzhou urban, male 490, female 463.734 children from Guangzhou rural area, male 375, female 359. The every subject was once done throat swabs culture every month, total 2,530 strains of group A streptococci were collected. The isolation and identification of GAS strains were assessed by conventional methods, T typing was performed by slide agglutination.Results The dominant isolates were T6 11.23%, T4 9.29%, T1 7.79%, T11 5.61%,T8/25/IMP19 5.42% and T5/27/44 4.98% respectively, non-typeable strains were 17.68%. There were statistical difference for the dominant GAS strains T typing between urban and rural area in Guangzhou(P<0.001). the dominant GAS strains in urban were T6 20.45%, T1 16.29%, T4 9.38% and T5/27/44 6.63% ,which were reached 52.75% in all GAS strains in urban; then main GAS strains T typing in rural area were T11 9.29%, T4 9.22%, T8/25/IMP19 8.55%, T6 4.61% and T5/27/44 3.79% respectively; 424 GAS strains were performed opacity factor(OF) test, 65.8% GAS strains were of negative. The dominant GAS strains varied during different years.Conclusions The dominant prevalent strains of GAS were T6, 4, 1,11,8/25/IMP19 and 5/25/44 in Guangzhou elementary schoolchildren P.R.C. between 1988 and 1994, T non-typeable was 17.68%;There obviosly statistical difference for T typing of the dominant GAS strains between urban and rural area in Guangzhou P.R.C.

关 键 词：广州市 儿童 A族链球菌T型 调查 流行病学 

分 类 号：R378.1[医药卫生—病原生物学] R181.81[医药卫生—基础医学]