金粉蕨素对脂质过氧化损伤内皮细胞的保护作用  

Protective effects of onychin on oxidative injured endothelial cells

作　　者：郭玉[1] 严鹏科[1] 朱炳阳[1] 

机构地区：[1]南华大学药物药理研究所

出　　处：《中国临床药理学与治疗学》2004年第6期612-614,共3页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：湖南省卫生厅中医药科研基金项目 (№ 2 0 0 0 0 0 6)

摘　　要：目的 :探讨金粉蕨素对脂质过氧化损伤人内皮细胞株 (ECV30 4 )的保护作用及其可能的分子作用机制。方法 :在铜离子诱发的低密度脂蛋白氧化修饰的基础上 ,建立内皮细胞 (endothelialcell ,EC)的脂质过氧化损伤模型 ;采用MTT比色法、硝酸还原酶法等方法 ,测定不同浓度金粉蕨素 (1.0、5 .0、10 .0 μmol·L-1)对EC生长抑制率、硫代巴比妥反应物 (thiobarbituricacid reactivesubstances ,TBARS)、LDH及NO含量的影响。结果 :氧化修饰低密度脂蛋白 (oxidativelymodifiedlowdensitylipoprotein ,ox LDL)作用 2 4h后 ,对EC的生长增殖有明显的抑制作用 ,细胞培养液中TBARS、LDH水平显著升高 ,NO含量降价 ;而不同浓度金粉蕨素与oxLDL共同孵育2 4h ,呈剂量依赖性对抗oxLDL对EC的损伤 ,并促进EC增殖 ,同时使培养液中TBARS、LDH含量降低 ,NO含量回升。结论 :脂质过氧化能直接损伤内皮细胞 。AIM: To investigate the protective effects of onychin on oxidative injured endothelial cells and its protective mechanism. METHODS: The injured model was established by endothelial cell treated with oxLDL. Protective effect of onychin on growth inhibition of endothelial cells injured by oxLDL was determined by MTT assay; NO concentrations in the medium were determined by nitrate reductase assay. The damage degree of endothelial cells was assessed by showing the TBARS and LDH levels in conditioned media. RESULTS: Onychin with various concentrations, given 24 hours, could decrease the growth inhibitory rates of endothelial cells injured by oxLDL, increase NO concentration and decrease the TBARS and LDH levels in the medium. CONCLUSION: Onychin can protect endothelial cells against oxidative damage.

关 键 词：脂质过氧化 金粉蕨素 内皮细胞 硫代巴比妥反应物 一氧化氮 乳酸脱氢酶 

分 类 号：R965.1[医药卫生—药理学] R966[医药卫生—药学]