氢化可的松抑制Jurkat细胞的调节性体积减小  

Inhibitory effect of hydrocortisol on regulatory volume decrease in Jurkat cells

作　　者：康劲松[1] 姜平[2] 冯娟[2] 张海龙[3] 李志昌[3] 汤富磊[3] 范少光[2] 祝世功[1] 宋德懋[2] 

机构地区：[1]吉林大学基础医学院病理生理教研室 [2]北京大学医学部生理学与病理生理学系,北京100083 [3]北京大学医学部

出　　处：《中国临床药理学与治疗学》2004年第6期615-618,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目 (№ 3 0 2 712 0 9)

摘　　要：目的 :研究氢化可的松对Jurkat细胞调节性体积减小 (regulatoryvolumedecrease ,RVD)的影响及可能的机制。方法 :通过MoticImagesAdvanced 3.1软件系统实时监测细胞体积的变化 ,并通过3 H掺入实验观察Jurkat细胞增殖。结果 :氢化可的松对Jur kat细胞RVD具有剂量依赖性的抑制作用 ,在 10 -4和 10 -3 mol·L-1时可以明显抑制Jurkat细胞的RVD ,而在 10 -9、10 -7和 10 -5mol·L-1时对Jurkat细胞的RVD没有明显影响。钾通道阻断剂奎宁 (quinine)亦可以抑制Jurkat细胞的RVD。氢化可的松和奎宁在10 -3 mol·L-1均可明显抑制Jurkat的增殖 (包括自然增殖和ConA诱导增殖 )。结论 :氢化可的松对JurAIM: To study the effect of hydrocortisol on regulatory volume decrease (RVD) in Jurkat cells and its possible mechanism. METHODS: The measurement of Jurkat cell volume was performed under the microscope and the imaging analysis which was carried out by Motic Images Advanced 3.1 Software. The cell proliferation was measured with 3H-TdR incorporation. RESULTS: Hydrocortisol (10 -4 and 10 -3 mol·L -1) inhibited RVD in Jurkat cells with dose dependent manner. A potassium channel blocker (quinine) was also found to have an inhibition to Jurkat cell RVD. The Jurkat cell proliferation (including with and without Con A) was significantly suppressed by hydrocortisol (10 -3 mol·L -1) and quinine (10 -3 mol·L -1). CONCLUSION: The inhibition of Jurkat cell RVD induced by hydrocortisol might be from the inhibition of potassium channel.

关 键 词：氢化可的松 JURKAT细胞 细胞体积调节性减小 钾通道阻断剂 淋巴细胞转化 

分 类 号：R977.11[医药卫生—药品]