哌芳安他对异丙肾上腺素诱发的小鼠心肌缺血的保护作用  

Protective effects of pivanampeta on myocardial ischemia induced by isoproteronol in mice

作　　者：余保瑞[1] 龙超良[1] 夏云峰[2] 刘同库[3] 汪海[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850 [2]解放军第304医院,北京100037 [3]北华大学医学院

出　　处：《中国临床药理学与治疗学》2004年第6期637-640,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：军队医药卫生"十五"重点项目 (№ 0 1Z0 2 5 )

摘　　要：目的 :评价新化合物哌芳安他 (pivanampeta)对异丙肾上腺素诱发的小鼠心肌缺血损伤的保护作用。方法 :昆明种小鼠 4 9只 ,雌雄兼用 ,随机分成 5组 ,每组 8～ 11只 ,灌胃给药 ,共 7d。正常对照组及损伤模型组 :按 10ml·kg-1给予生理盐水 ,每日 2次 ;哌芳安他低、中、高剂量组 :分别按 1,5 ,2 5mg·kg-1给予哌芳安他 ,每日 2次 ,自d 6第 1次给药后 0 .5h内 ,损伤模型组和哌芳安他高中低剂量组动物连续 2d接受皮下注射异丙肾上腺素 (iso proteronol,ISO) ,每次剂量 2 0mg·kg-1,每日 1次 ,正常对照组给予皮下注射等容积生理盐水 (NS) ,每日1次。各组动物于末次皮下注射异丙肾上腺素或生理盐水后 2h ,采血测定磷酸肌酸激酶 (CK)及乳酸脱氢酶 (LDH) ,留取心脏观察心肌组织形态学变化。结果 :与正常对照组相比 ,ISO组血清LDH及CK水平显著升高 (P <0 .0 1) ,组织形态学改变明显 ,心肌细胞损伤严重。各哌芳安他用药组血清LDH及CK水平较ISO组有所降低 (P <0 .0 5 ) ,组织细胞损伤明显减轻。结论AIM: To evaluate the protective effects of pivanampeta on myocardial ischemia induced by isoproteronol (ISO) in mice. METHODS: Forty-nine Kunming mice were divided into five groups randomly, each group including 8-11 mice and administered for 7 days. NS in control and ISO groups were given NS 10 ml·kg -1 twice a day. The three doses of pivanampeta ( 1.0, 5.0, and 25.0 mg·kg -1) in treatment groups were given by ig twice a day respectively. In the last two days, ISO was used at 20 mg·kg -1 sc half an hour after NS or pivanampeta were administered once a day in ISO and treatment groups, while NS was injected subcutaneously in control group continuously. Two hours after last subcutaneous administration of ISO or NS, the blood and hearts of mice were obtained to measure the serum levels of LDH and CK and to investigate the morphological changes of myocardium, respectively. RESULTS: Compared with the control group, the levels of LDH and CK in ISO groups were increased significantly (P< 0.01), and morphologically severe injury was found in myocardial tissue and cells. And in each treatment group, the levels of LDH and CK were reversed (P< 0.05), and myocardial injury reduced in comparison with that of ISO group. CONCLUSION: Pivanampeta has exact protective effect on myocardial ischemia mice induced by ISO in a dose-dependent manner.

关 键 词：心肌缺血 异丙肾上腺素 哌芳安他 小鼠 

分 类 号：R965.2[医药卫生—药理学]