脱氧核酶抑制耐甲氧西林金黄色葡萄球菌耐药基因的表达  

DNAzymes inhibit the expression of Methecillin-resistant staphlococcus aureus drug-resistant genes

作　　者：刘杰[1] 罗晓星[1] 孟静茹[1] 扈本荃[1] 王海芳[1] 孟嘉[1] 

机构地区：[1]第四军医大学药理学教研室

出　　处：《中国临床药理学与治疗学》2004年第6期641-645,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目 (№ 3 0 2 715 5 6);全军医药卫生科研基金项目(№ 0 2M0 0 8)

摘　　要：目的 :探讨抑制耐甲氧西林金黄色葡萄球菌(MRSA)耐药基因blaR1表达的脱氧核酶对MRSA耐药性的影响。方法 :以耐药基因blaR1的mRNA为靶点 ,设计合成脱氧核酶DrzB ,导入细菌后通过平板克隆形成实验观察DrzB对MRSA细菌耐药性的影响 ,通过RT PCR观察DrzB对耐药基因blaR1表达的抑制作用。结果 :加入DrzB后培养的MRSA ,在含苯唑西林 (6mg·L-1)的M H琼脂培养基表面生长的菌落单元计数低于对照组 (P <0 .0 1) ,blaR1的mRNA表达水平也较对照组降低 (P <0 .0 1)。结论 :导入以blaR1的mRNA为靶基因的DrzB阻断耐药基因表达 ,可以部分恢复MRSA的抗生素敏感性。脱氧核酶DrzB是一种特异性的。AIM: To evaluate the inhibition effects of DNAzymes specific to Methecillin-resistant staphlococcus aureus (MRSA) drug-resistant gene blaR1 on the expressions of drug-resistance. METHODS: mRNA of blaR1 was chosen as a target gene and DNAzyme DrzB specific to it was designed and synthesized. After DrzB was introduced into the MRSA, drug-resistant characters of MRSA were evaluated by cloning efficiency. The inhibition effects of DrzB on the expressions of drug-resistant gene blaR1 were observed by RT-PCR in MRSA. RESULTS: Colony forming units (CFU) of MRSA incubated with DrzB on the M-H agar added oxa ( 6 mg·L -1) were less than those of control group (P< 0.01). Levels of blaR1 mRNA of the DrzB group were lower than those of the control group (P< 0.01). CONCLUSION: Antibiotic sensitivity on MRSA can be partially restored by exogenously delivered DrzB targeted to mRNA of blaR1 in blocking drug-resistant genes expression. DNAzyme DrzB is proved a specific and effective gene therapeutic means.

关 键 词：耐甲氧西林金黄色葡萄球菌 耐药性 基因 表达 脱氧核酶 抑止效应 

分 类 号：R965.1[医药卫生—药理学]