阿霉素热化疗诱导A549细胞凋亡及线粒体跨膜电位的变化  

Apoptosis induced by thermochemotherapy of adriamycin (ADM) and the change of mitochondial transmembrance potential in A549 cells

作　　者：张继贤[1] 张启昆[1] 万毅新[1] 

机构地区：[1]兰州医学院第二附属医院呼吸内科

出　　处：《中国临床药理学与治疗学》2004年第6期662-665,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的 :探讨阿霉素 (adrimycin ,ADM)热化疗诱导人肺腺癌A5 49细胞凋亡及对线粒体跨膜电位(Δψm)的影响。 方法 :不同浓度的阿霉素作用于体外培养的A5 49细胞 ,4 2 .5℃作用 30min后 ,37℃继续培养 ,在不同时间内检测。用电镜、MTT法、流式细胞仪检测。结果 :阿霉素热化疗明显增强对A5 49细胞的抑制作用 ,细胞内阿霉素的浓度显著高于化疗组 (P <0 .0 1) ;阿霉素浓度为 1.0 ,2 .0mg·L-1时 ,热化组与化疗组比较 ,凋亡率增高 ,线粒体膜电位下降 (P <0 .0 1)。结论 :线粒体跨膜电位下降可能是ADM热化疗诱导A5AIM: To investigate the effect of apoptosis and the change of mitochondial tansmembrane potential in A549 cells induced by thermochemotherapy of ADM. METHODS: ADM of different concentrations was directly applied to A549 cells cultivated in vitro. Cells maintained at 42.5 ℃ for 30 min. RESULTS: The inhibition of A549 cells was increased by thermochemotherapy of ADM significantly,and the cytoplasmic concentration of thermochemotherapy of ADM was significantly higher than that of chemotherapy (P< 0.01). When it was 1.0 and 2.0 mg·L -1, apoptosis rate was increased and mitochondrial transmembrane potential was decreased by thermochemotherapy of ADM (P< 0.01). CONCLUSION: The change of motochondial transmembrane potential was the key of apoptosis induced by thermochemotherapy of ADM in A549 cells.

关 键 词：阿霉素 热化疗 A549细胞 凋亡 线粒体 跨膜电位 

分 类 号：R453.2[医药卫生—治疗学] R963[医药卫生—临床医学]