苯丙酸诺龙对烧伤大鼠创面成纤维细胞的影响  

Effects of Nandrolone Phenylpropionate on Fibroblasts after Injury in Rats

作　　者：岑瑛[1] 刘宁[2] 刘晓雪[1] 李凯[1] 

机构地区：[1]四川大学华西医院烧伤整形科,成都610041 [2]天津市第一中心医院烧伤整形科

出　　处：《四川大学学报（医学版）》2004年第4期508-511,共4页Journal of Sichuan University(Medical Sciences)

摘　　要：目的　探讨苯丙酸诺龙 (NP)对烧伤后大鼠创面成纤维细胞 (FB)增殖及其胶原合成的影响。方法　肉芽创面原代成纤维细胞培养 ,测定不同剂量的 NP对 FB细胞存活数和增殖指数的影响 ;采用 2 0 %总体表面积深 度烧伤大鼠模型 ,随机分为治疗组 (NP)和对照组 (C) ,分析比较两组间成纤维细胞雄激素受体 (AR)和 α1 ( )前胶原蛋白 m RNA的表达及其相关关系。结果　 1各 NP组 A值均高于同期对照组 ;流式细胞术检测 FB增殖指数 (PI)示 NP组的 PI高于同期对照组 ,有显著性差异 (P<0 .0 5 )。 2 NP组第 4、7、1 4和 2 1 d的 α1 ( )前胶原蛋白m RNA表达均高于同期对照组 ,第 4 d两组相比无显著性差异 ,其余均有显著性差异 (P<0 .0 5 )。3在各时间点 NP组成纤维细胞 AR的表达均明显高于对照组 ,有显著性差异 (P<0 .0 5 )。 4 AR的表达与胶原蛋白基因的表达之间呈正相关关系 (r=0 .836 )。结论　蛋白同化激素 NP能够促进 FB的复制 ,且有剂量和时间依赖性 ;NP在转录水平促进 I型前胶原蛋白 m RNA表达 ,这与成纤维细胞Objective To explore the effects of nandrolone phenylpropionate (NP) on fibroblasts after injury in rats. Methods Thirty two Wistar rats with a deep second degree scald injury and 20% total body surface area were randomly divided into two groups to receive either 5 mg/kg NP (NP group) or normal saline as placebo (control group) every other day. Integrated optical density(IOD)of androgen receptor (AR) and mRNA expression level of α1(Ⅰ) procollagen were measured by immunohistochemistry and quantitive fluorescent RT PCR individually on the post burn days 4,7,14 and 21. Fibroblasts were isolated from granulation tissue of a Wistar rat and were cultured in RPMI1640 with the addition of NP at different concentrations. Cell viability of fibroblasts was measured by MTT test, and the proliferative index, by flow cytometry(FCM). Results ① Compared with the A value of the concurrent control group, higher A values were seen in NP groups containing different concentrations of NP, and significant difference of proliferative index was observed in NP group, P <0 05. ② The expression of α1(Ⅰ) procollagen mRNA in NP groups was much higher than that in control groups. A significant difference between the two groups was noted on the post burn days 7,14 and 21, P <0.05, but no difference was seen on day 4. ③ The density of AR on fibroblasts in NP group was higher than that in control group at each time point. And a positive correlation between the expression of α1(Ⅰ) procollagen mRNA and the quantity of AR on fibroblasats was confirmed( r =0.836). Conclusion Nandrolone phenylpropionate could promote fibroblast replication, increase the mRNA level of α1(Ⅰ) procollagen and enhance the density of AR on fibroblasts.

关 键 词：苯丙酸诺龙 细胞存活数 增殖指数 雄激素受体 α1(Ⅰ)前胶原蛋白mRNA 

分 类 号：R644[医药卫生—外科学]