大黄高效液相色谱法指纹图谱分离条件的优化  被引量:10

Optimization of the Separation Conditions of Fingerprint for Rhubarb by High Performance Liquid Chromatography

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作  者:徐雄良[1] 张志荣[1] 黄园[1] 

机构地区:[1]四川大学华西药学院靶向药物与新型给药系统重点实验室,成都610041

出  处:《四川大学学报(医学版)》2004年第4期559-562,共4页Journal of Sichuan University(Medical Sciences)

基  金:四川省重点科技项目 (批准号 :0 1SG0 0 8-0 1)资助

摘  要:目的 优化大黄高效液相色谱法指纹图谱 (HPL C- FPs)的分离条件。方法 以 RP- HPL C法 ,使用Shim- pack CL C- ODS柱 (6 .0 m m i.d.× 1 5 0 m m,5μm ) ,流动相为甲醇 - 1 m l/ L磷酸溶液 ,检测波长为 2 5 4 nm ,柱温为 4 0℃ ,考察大黄总提取物在不同梯度洗脱条件下的分离情况。结果 优选出的大黄 HPL C- FPs分离条件 ,可使大黄总提取物中各组分达到较佳分离 ,稳定性、精密度、重现性好。结论 该色谱分离条件可用于建立大黄药材HPL C-Objective To optimize the separation conditions of fingerprint for Rhubarb by high performance liquid chromatography and lay the foundation of studies on its fingerprint by high performance liquid chromatography. Methods The reversed phase high performance liquid chromatographic method was adopted. The samples were extracted by ultrasound and separated by a linear gradient elution on Shim pack CLC ODS column (6.0 mm i.d.×150 mm, 5 μm), and then, with the methanol 1 ml/L phosphoric acid solution as mobile phase, were detected at UV 254 nm and column temperature 40 ℃. During the linear gradient elution program, the volume fraction of methanol in mobile phase changed as follows: 0 min 5 min, 20%; 5 min 25 min, from 20% to 50%; 25 min 35 min, from 50% to 80%; 35 min 60 min, from 80% to 90%; 60 min 70 min, 90%. The flow rate was 0.8 ml/min from 0 min to 35 min, 0.6 ml/min from 35 min to 50 min and 1.0 ml/min after 50 min. Results The separation conditions described above achieved good result in the aspects of separable effect, stability, sensitivity, and reproducibility. Conclusion The optimum separation conditions of fingerprint would be useful for establishing the method of fingerprint for Rhubarb by high performance liquid chromatography.

关 键 词:大黄 高效液相色谱法 指纹图谱 分离条件 

分 类 号:R284[医药卫生—中药学]

 

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