不同人群血清对人SARS冠状病毒和动物SARS样冠状病毒反应性的比较  

Comparison of antibody reaction against human SARS-CoV and animal SARS-CoV-like viruses in SARS case and animal market worker with SARS-CoV-like IgG positive

作　　者：徐慧芳[1] 王鸣[1] 高阳[1] 郑伯健[2] 顾菁[1] 秦鹏哲[1] 张周斌[1] 邹晓忠[3] 梁彩云[1] 赵宇腾[1] 高凯[1] 

机构地区：[1]广州市疾病预防控制中心,广东广州510080 [2]香港大学 [3]广州市白云区疾病预防控制中心

出　　处：《华南预防医学》2004年第3期4-6,共3页South China Journal of Preventive Medicine

基　　金：广东省医学科学技术研究基金项目 (A2 0 0 45 78)

摘　　要：目的　探讨不同人群血清中人SARS冠状病毒抗体和动物SARS样冠状病毒抗体所表达的意义及其之间关系。方法　应用人SARS冠状病毒和动物SARS样冠状病毒抗原片进行间接免疫荧光 (IFA)定性和半定量试验 ,检测 2 3份一般人群、2 5份动物市场从业人员和 74份SARS病人的SARS冠状病毒IgG抗体阳性 (ELISA法检测 )血清样本 ,并进行比较分析。结果　 2 3份一般人群、2 5份动物市场从业人员和 74份SARS病人的血清样本的IFA阳性率分别为 73 91 %、6 8 0 0 %和1 0 0 0 0 % ,在IFA阳性人群中其血清样本既能对人SARS冠状病毒也能对动物SARS样冠状病毒产生反应的分别占 1 0 0 0 0 % (1 7/ 1 7)、6 4 71 % (1 1 / 1 7)和 94 5 9% (70 / 74 ) ;70 5 9% (1 2 / 1 7)一般人群和76 4 7% (1 3/ 1 7)动物从业人员其血清中的动物SARS样冠状病毒抗体水平高于人SARS冠状病毒 ;而SARS病人血清中的人SARS冠状病毒与动物SARS样冠状病毒抗体水平以两者相同和前者高于后者为多 ,分别占 5 6 76 % (4 2 / 74 )和 33 78% (2 5 / 74 )。结论　不同人群血清中的人SARS冠状病毒抗体和动物SARS样冠状病毒抗体两者之间均存在着一定的交叉反应现象 ,从血清学上提示了人SARS冠状病毒与动物SARS样冠状病毒之间存在着很高的同源性 ;人SARS冠状?Objective To compare the reaction and level of antibody against human SARS CoV and animal SARS CoV like viruses in SARS case, animal market worker and control with SARS CoV like Ig G positive. Methods 122 serum samples, with IgG positive against SARS CoV, were collected , 74 from SARS patients, 25 from wild animal market workers and 23 from control. Antibody against human SARS CoV and animal SARS CoV like virus was detected by qualitative and semi quantitative Immunofluorescence assay(IFA). Results The percentages of antibody both positive against human and animal SARS CoV were 94 59%(70/74) in SARS case, 44%(11/25) in wild animal market worker and 73 91%(11/23) in control. The levels of antibody against animal SARS CoV like viruses were higher than that against human SARS CoV in wild animal market worker and control. Conclusion The result suggested there was a high homogeneity between human SARS CoV and animal SARS CoV like viruses and qualitative and semi quantitative Immunofluorescence assay (IFA) may provide the evidence for diagnosis.

关 键 词：严重急性呼吸综合征 冠状病毒属 抗体 荧光抗体技术 间接 

分 类 号：R563.1[医药卫生—呼吸系统]