组织工程化肌腱体外构建的环境优化及系统设计  

Environmental optimization and system design for tendon engineering in vitro

作　　者：李宏[1] 魏娴[1] 刘伟[1] 崔磊[1] 曹谊林[1] 

机构地区：[1]上海第二医科大学第九人民医院整形外科

出　　处：《中华创伤骨科杂志》2004年第7期778-780,共3页Chinese Journal of Orthopaedic Trauma

基　　金：国家高技术研究发展计划(863计划)课题(2002AA205031)

摘　　要：目的设计一套构建具有一定功能和形态的肌腱组织的体外培养方法。方法根据肌腱细胞体内的生物力学环境,在细胞的培养过程中,设计一套能够提供张力和参数测量系统的生物反应器,在肌腱的培养过程中对肌腱施加不同形式的张应力作用,以期在体外获得力学性能优化的自体肌腱。结果细胞培养和张应力控制系统是由完全透明的有机材料制成的,整个系统由细胞培养室、测量系统和排换液系统等部分构成,在长期的肌腱细胞培养中,可提供肌腱-材料支架动态的培养方式。细胞培养室能很方便与气动肌腱连接,产生持续的脉冲张力作用。结论用肌腱生物反应器可以使肌腱组织复合物在形成过程中始终处于力学环境中,促进肌腱组织中胶原纤维的平行排列,提高肌腱的力学性能,为组织工程化肌腱的产业化提供一种新的方法。Objective To establish a culture system with optimized environment for tendon engineering in vitro. Methods The device was designed according to biomechanical data of tendon tissue. Tensile force was exerted on the engineered tendon in different forms to optimize the biomechanical environment and measured for meticulous modulation. Results The whole device consisted of culture chamber, control system, measure system, and media exchange system. The culture chamber made from Plexiglas was transparent and provided continuous pulses of tensile force controllable and measurable. Conclusion Since the engineered tendon placed in such a bioreactor can receive optimal mechanical stimuli, the device can result in better alignment of collagen fibers and mechanical properties. The method will shed new light on industrialization of tendon engineering.

关 键 词：组织工程 生物反应器 张力 肌腱 

分 类 号：R318.1[医药卫生—生物医学工程]