磺化标记的DNA探针用于检测恶性疟感染的评价  

Evaluation of Sulfonated DNA Probe (Sulfoprobe) for Diagnosis of Plasmodium falciparum

作　　者：缪为民[1] 管惟滨[1] 徐晓春[1] 周元昌[1] 陆德如[2] 董蓓华 陈蕊雯[2] 

机构地区：[1]第二军医大学寄生虫学教研室,200433 [2]第二军医大学分子遗传学教研室,200433 [3]第二军医大学免疫学教研室,200433

出　　处：《第二军医大学学报》1993年第2期107-111,共5页Academic Journal of Second Military Medical University

摘　　要：采用非同位素磺化修饰法(sulfomodification),标记恶性疟原虫DNA重组质粒片段pPF14。用此探针,以斑点杂交试验检测恶性疟原虫感染,并和常规血片法进行比较。结果显示:对于体外培养的恶性疟原虫,该法可检出25 pg纯化的DNA,或0.001％的原虫率。探针和人白细胞、鼠伯氏疟原虫、约氏疟原虫DNA均无交叉反应。对179份云南省西双版纳自治州疟疾病人血样进行检测,探针法结果和血片法有较好的相关性,探针法检测恶性疟的符合率为92.5％(99/107),和间日疟的交叉反应率为1.39％(1/72),和48例正常人血无一交叉反应。In this study, the DNA probe pPF14 was nonradioactively labelled by sulfo-modification, and used in a dot blot hybridization assay for detection of P. falciparum. The results showed that the sulfoprobe successfully detected 25 pg purified DNA and 0. 001% parasitemia of cultured P. falciparum, and did not react with human leukocyte DNA. In the study of 179 clinical blood samples of malaria patients from Yunnan province, the DNA probe results correlated well to blood smear ones. Of 107 P. falciparum samples determined by microscope examination, 99 were positive by sulfoprobe (925%), while of 72 P. vivax samples, 1 was positive; no cross reactions were found with any of 48 normal blood samples. It is indicated that sulfoprobe may be useful in specific diagnosis and epidemiological investigation of P. falciparum infection.

关 键 词：DNA 探针 磺化修饰 疟疾 

分 类 号：R531.304[医药卫生—内科学]