毛细管电泳测定三精双黄连口服液中的黄芩甙元、黄芩甙、绿原酸和咖啡酸  

Determination of Baicalein,Baicalin,Chlorogenic Acid and Caffeic Acid in Sanjing Shuanghuanglian Oral Liquid by Capillary Electrophoresis

作　　者：吕元琦[1] 邬春华[1] 袁倬斌[1] 

机构地区：[1]中国科学院研究生院化学系,北京100039

出　　处：《分析测试学报》2004年第4期98-100,共3页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20175025;29875027;20235010)

摘　　要：建立了毛细管电泳法测定中药复方制剂双黄连口服液中黄芩甙元、黄芩甙、绿原酸和咖啡酸的方法,通过研究缓冲溶液pH和浓度、分离电压、进样时间和有机添加剂的影响优化了分析条件。在优化的条件下,20min内实现了4种物质的良好分离。黄芩甙元、黄芩甙、绿原酸和咖啡酸峰高和质量浓度分别在0.05～1.50、0.06～1.20、0.02～0.50和0.02～0.50g／L范围内呈良好线性;检出限分别为0.015、0.020、0.004、0.004g／L。基于迁移时间和峰高的重复性分别为:黄芩甙元,1.70%和3.94%;黄芩甙,1.60%和3.63%;绿原酸,1.60%和2.05%;咖啡酸,1.51%和2.83%。通过分析实际样品并做加标回收实验验证了该方法的可行性。A capillary electrophoretic method has been developed for the determination of baicalein,baicalin,chlorogenic acidandcaffeic acidinTraditional Chinese Medicinal preparationSanjingShuanghuanˉglian oral liquid.The analytical conditions were optimized after investigating the effects of buffer pH and concentration,applied voltage,injection time and organic modifier.Under optimized conditions,the above four analytes were separated in20min.A good linear relationship between the peak height and conˉcentration was observed in the ranges of0.05～1.50,0.06～1.20,0.02～0.50and0.02～0.50g/L for baicalein,baicalin,chlorogenic acid and caffeic acid,respectively,whereas the detection limits based on signal levels three times of noise levels were found to be0.015,0.020,0.004and0.004g/L for these four analytes,respectively.The reproducibilities based on migration time and peak height were1.70%and3.94%for baicalein,1.60%and3.63%for baicalin,1.60%and2.05%for chloroˉgenic acid,1.51%and2.83%for caffeic acid.The method has been verified by the recovery experiˉments of spiked real samples.

关 键 词：毛细管电泳 黄芩甙元 黄芩甙 绿原酸 咖啡酸 

分 类 号：O6578[理学—分析化学] TQ460.72[理学—化学]