菊花叶片离体高效再生体系的建立  

ESTABALISHMENT OF HIGH EFFICIENT REGENERATION SYSTEM FROM LEAVES OF CHRYSANTHEMUN

作　　者：刘军[1] 赵兰勇[1] 丰震[1] 张美蓉[1] 韩进[1] 杨传强[1] 

机构地区：[1]山东农业大学林学院,山东泰安271018

出　　处：《山东农业大学学报（自然科学版）》2004年第2期177-182,共6页Journal of Shandong Agricultural University：Natural Science Edition

摘　　要：本文报道了以菊花叶片为外植体 ,诱导植株再生 ,建立高效植株再生体系。获得诱导愈伤组织、芽和根的最佳培养基。在有 6 -BA、NAA的MS培养基上获得较高的愈伤组织诱导率 ,在有 2 ,4 -D的培养基上其愈伤组织诱导率很低。带有愈伤组织的外植体转移到有 6 -BA、NAA的MS培养基上催化芽的产生 ,并筛选出能直接诱导芽再生的培养基 (MS +6 -BA(3mg/L) +NAA(1mg/L) )。AgNO3 可明显提高芽的再生率。茎段在 1/ 2MS和有IBA或NAA的 1/ 2MS培养基上诱导生根。生根的小苗在温室里生长良好。A regeneration system from leaves of chrysanthemum (Dendranthema morifolium) was developed. The optimum media were obtained for callus, shoot regeneration and shoot rooting. On the media with 6-BA, NAA and 2,4-D, calli were induced from leaf explants. After explants with calli were transferred onto MS media with 6-BA and NAA to trigger shoot regeneration, adventitious shoots were regenerated from leaf explants of chrysanthemum, and the medium(MS+6-BA(3mg/L)+NAA(1mg/L)), which could directly induce shoot regeneration, was selected. AgNO3 enhanced shoot induction rate. Shoots were rooted on half-strength MS media with or without IBA or NAA. The rooted plantlets survived in greenhouse.

关 键 词：菊花 叶片离体 再生体系 外植体 组织培养 培养基 愈伤组织 

分 类 号：S682.11[农业科学—观赏园艺]