高压氧水飞蓟宾联合应用对兔肢体缺血再灌注损伤的治疗作用  

Experimental Study of Effectiveness of Hyperbaric Oxygen Combined with Silybin on Lschemic-reperfused Injured Limbs

作　　者：康一凡[1] 高建章[1] 方以群[2] 芮耀诚[3] 孙笃新[3] 曾国钱[3] 

机构地区：[1]长海医院骨科,200433 [2]海军医学研究所,上海200433 [3]第二军医大学药学院,200433

出　　处：《第二军医大学学报》1993年第1期44-47,共4页Academic Journal of Second Military Medical University

摘　　要：27只家兔分成假手术组、对照组、水飞蓟宾组、高压氧组、高压氧水飞蓟宾联合组。以一侧后肢造成缺血再灌注损伤模型,缺血6 h,再灌注1 h,分别取股静脉回流血测定丙二醛、SOD;胫前肌标本测ATP、CP及进行超微结构检查。结果显示应用高压氧与水飞蓟宾联合组效果最好,与对照组相比,丙二醛值下降明显,SOD、ATP、CP值显著提高。认为高压氧不加重再灌注时脂质过氧化损害,并能使线粒体大量再生,增加细胞能量供应,促进损害组织恢复。水飞蓟宾能增高SOD活力,降低氧自由基损害,两者联合应用,有协同作用。27 rabbits were divided into normal group(n = 4),control group (n = 5), silybin group (n = 6), hyperbaric oxygen (HBO) group (n = 6), and HBO combined with silybin group (n = 6). Circulation of hindlimb was interrupted completely for 6 h and reperfused for 1 h. Malondialdehyde (MDA) and superoxide dismutase(SOD) in plasma from ischemic-reperfused injured limb were measured. Adenosine triphosphate (ATP) and creatine phosphate (CP) in samples taken from anterior tibial muscle were determined. Ultrastructural changes of injured muscle were observed. The results showed that HBO combined with silybin treatment had a favourable effect on ischemic and reperfused injured limb muscle with reduction of the lipid peroxidated injury, increase of the SOD activity and the high energy phosphate compounds (ATP and CP), and a promoting recovery of injured muscle cells. HBO and silybin had a synergistic action. It suggests that HBO combined with silybin is an effective method for treatment of ischemic and reperfused injured limb.

关 键 词：再灌注损伤 高压氧 水飞蓟宾 缺血 

分 类 号：R680.5[医药卫生—骨科学]