cAMP和cGMP对棉铃虫神经细胞高电压激活钙通道的调节作用(英文)  

Modulations by cAMP and cGMP of the high-voltage activated Ca^(2+) channels in the central neurons of the cotton bollworm, Helicoverpa armigera

作　　者：李杰[1] 杜育哲[1] 佟帆[1] 贺秉军[1] 刘安西[1] 

机构地区：[1]南开大学生命科学学院

出　　处：《昆虫学报》2004年第4期429-433,共5页Acta Entomologica Sinica

基　　金：国家自然科学基金项目 ( 3 0 2 70 884,3 0 10 0 119)

摘　　要：用全细胞膜片钳法研究了cAMP和cGMP对棉铃虫Helicoverpaarmigera 3龄幼虫胸腹神经节细胞高电压激活钙通道的调节作用。细胞外液中加入腺苷酸环化酶 (AC)激活剂福斯克林 (forskolin) 0 .1mmol L ,对于Ba2 + 介导的钙通道电流激活电压、峰电压、峰电流变化以及通道激活和电流达到峰值的时间无影响。电极内液中加入 1mmol L的cGMP则明显抑制峰电流 ,且抑制作用呈时间依赖性和浓度依赖性 ,而对激活电压、峰电压无影响。结果提示 ,棉铃虫神经细胞高电压激活钙通道的活动可能不受细胞内cAMP水平提高的影响 。The effects of cAMP and cGMP on the high-voltage activated (HVA) Ca^(2+) channels in the central neurons isolated from the 3rd instar larvae of the cotton bollworm (Helicoverpa armigera) were studied using the whole-cell patch clamp technique. With barium as current carrier, the currents of Ca^(2+) channels were recorded by blocking Na^+ and K^+ currents. Extracellular application of 0.1 mmol/L forskolin, a stimulator of adenylyl cyclase (AC), had no effect on the peak amplitude of Ba^(2+) currents. In contrast, 1 mmol/L cGMP in the intracellular solution suppressed the current amplitude significantly and the inhibition was time- and dose-dependent. The results suggest that the activities of HVA Ca^(2+) channels in the cotton bollworm may not be affected by the elevated cAMP, but can be inhibited by the increased cGMP.

关 键 词：棉铃虫 环磷酸腺苷 环磷酸鸟苷 高电压激活钙通道 膜片钳 

分 类 号：Q965[生物学—昆虫学]