FISH方法在辐射事故评介中的应用  

Application of FISH method in evaluation of a radiation accident

作　　者：王明明[1] 陈英[2] 段志凯[3] 郑斯英[1] 张淑贤[3] 徐洪兰[3] 

机构地区：[1]苏州大学放射医学与公共卫生学院,苏州215007 [2]北京放射医学研究所,北京100850 [3]中国辐射防护研究院,太原030006

出　　处：《辐射研究与辐射工艺学报》2004年第4期237-242,共6页Journal of Radiation Research and Radiation Processing

基　　金：国防科工委基金(Y5573262)资助

摘　　要：为研究辐射远后危害效应,探讨荧光原位杂交(Fluorescence in situ hybridization,FISH)方法用于剂量重建的可行性。同时,用FISH、常规染色体畸变、G显带、细胞松弛素B(Cytochalasm B,CB)微核和次黄嘌呤鸟嘌呤磷酸核糖转移酶(Hypoxanthine guanine phosphoribosyl transferase,HPRT)基因位点突变分析方法对3名事故受照射者(F、S、Y)进行了照射后7年半的生物学终点检测,并对宫内受照射者S的生长发育指标进行了测量。FISH方法检测易位的结果显示,在观察足够数量细胞时用易位率估算的生物剂量与照射后模拟的物理剂量非常吻合,证明该方法可用于剂量回顾和剂量重建。从FISH、常规染色体畸变及G显带分析结果发现,受照射7年半后受检者体内仍有明显的染色体畸变,且与受照射剂量呈现良好的剂量依赖性。实验结果还表明,宫内受照射者S出生后的生长发育状况基本正常。To study effects of long term radiation hazard and explore the possibility of the application of chromosome aberration and FISH method to dose retrospection and reconstruction, FISH method was used to detect biological destination of three accidental victims at 7.5 years after Xinzhou accident. In the meantime, conventional chromosomal aberration, G- banding, CB micronuclei and HPRT gene locus mutation assays were performed. In addition, the growth and development of Victim S, who suffered the radiation accident as a fetus, were examined. And comparison of dose estimations between chromosome aberration and FISH method of the victims was conducted. The results demonstrated that the biological dose estimated by translocation frequency is very close to the imitated dose by the physical way after the accident if enough cells are observed. It is suggested that FISH may be applied to dose retrospection and reconstruction. Obvious chromosomal aberrations still existed in the examined victims at 7.5 years after the accident and displayed good dose correlative dependence. The results also showed that the growth and development of S were basically normal after birth.

关 键 词：荧光原位杂交 常规染色体畸变 事故受照射者 剂量估算 

分 类 号：R811.5[医药卫生—放射医学]