SOD对肿瘤细胞凋亡影响和抗氧化损伤的研究:Ⅲ.SOD对受分次照射的荷瘤小鼠肿瘤细胞脂质过氧化影响及分子机制  

The studies of effect on tumor cell apoptosis with SOD and its anti-oxidation effect Ⅲ. The effect of SOD on lipid peroxidation of tumor cell of tumor-bearing mice under fractional irradiation

作　　者：刘永彪[1] 赵杰[2] 刘颖[2] 郝兴芝[2] 王绪[2] 姚思德[1] 

机构地区：[1]中国科学院上海应用物理研究所辐射技术应用中心,上海201800 [2]徐州医学院肿瘤研究所,徐州221002

出　　处：《辐射研究与辐射工艺学报》2004年第4期243-248,共6页Journal of Radiation Research and Radiation Processing

基　　金：江苏省教委自然科学基金(98KJD320014)部分资助

摘　　要：以荷瘤小鼠为动物模型,研究了全身照射下注射超氧化物歧化酶(SOD)对肿瘤细胞脂质过氧化、肿瘤组织端粒酶活性、肿瘤原癌基因c-Jun蛋白表达的影响。研究发现,SOD能增加S180肉瘤及Lewis肺癌组氧化产物丙二醛(MDA)含量,降低S180肉瘤谷胱甘肽(GSH)含量及血清超氧化物歧化酶总活力(T-SOD)、匀浆谷胱甘肽过氧化物酶(GPX-Px)活力;降低Lewis肺癌组GPX-Px及T-SOD活力,不影响GSH含量;降低H22肝癌组GPX-Px的活力,不影响MDA、GSH含量及T-SOD活力。SOD明显降低接受电离辐射的S180肉瘤及H22肝癌荷瘤小鼠肿瘤组织中端粒酶的阳性表达率,减少原癌基因c-Jun蛋白在S180肉瘤、Lewis肺癌荷瘤鼠中的表达。这一辐射增敏作用因肿瘤的种类而不同,可能与SOD在不同肿瘤中对癌基因c-Jun蛋白表达和端粒酶活性影响及药物在不同器官的代谢差异有关。In this paper the effect of injury of SOD on lipid peroxidation of tumor cell, telomerase activities in tumor tissues, expression of protooncogene c-Jun pretein were been studied with irradiated tumor-bearing mice. It has been found that content of MDA increased in S180 sarcom group and Lewis lung cancer group in situation of SOD injury while the content of GSH, GPX and activity of T-SOD decreased For former and with no change of GSH for later. It has also been found that SOD can reduce the telomerase activities in S180 and H22 and expression of protooncogene c-Jun in S180 sarcom, Lewis lung cancer tumor tissues. The radiosensitivity of SOD may have some relationship with the effect on expression of protooncogene c-Jun and telomerase activities, and metabolism of SOD in different kinds of tumor.

关 键 词：超氧化物歧化酶 荷瘤小鼠 辐射敏感性 原癌基因C-JUN 端粒酶 

分 类 号：R811.5[医药卫生—放射医学]