HPLC法同时测定离体透皮实验接收液中萘替芬和酮康唑浓度  

Simultaneous HPLC Determination of Naftifine and Ketoconazole in vitro Percutaneous Solution

作　　者：何海霞[1] 柳青[1] 杨宇宏[2] 周远大[1] 

机构地区：[1]重庆医科大学附属第一医院,重庆400016 [2]重庆市沙坪坝区人民医院药剂科,重庆400030

出　　处：《药物分析杂志》2004年第3期264-267,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立HPLC法同时测定离体透皮实验接收液中萘替芬和酮康唑浓度。方法:氯雷他定作内标,透皮液经乙醚提取后,采用Hypersil C18柱(5μm,5.0 mm×200 mm),以乙腈-甲醇-水-三乙胺(35:40:25:0.8,用冰醋酸调至pH 7.0)为流动相,流速1.2 mL·min-1;在紫外265 nm处检测。结果:萘替芬和酮康唑线性范围分别为0.08040-2.010μg·mL-1(r=0.9936)和0.2016-5.040μg·mL-1(r=0.9999),最低检出浓度分别为0.0201μg·mL-1和0.0403μg·mL-1,不同浓度的平均回收率(n=15)分别为102.6%和100.1%。结论:本法灵敏度、准确度及回收率均能达到离体透皮实验接收液中药物浓度的测定要求,也可用于药代动力学研究。Objective:To establish a HPLC method for the simultaneous determination naftifine (NTF) and ketoconazole(KCZ)in vitro percutaneous solution. Methods: Loratedine (LORA) was used as internal standard. The percutaneous solution was extracted with ether. A Shimadzu HPLC instrument with Hypersil C18 column(5μm,5 mm×200 mm) was used. The mobile phase was a mixture of acetonitrile,methanol, water and triethylamune in a ratio of 35:40:25:0.8(adjusted pH to 7.0 by acetic acid) at a flow rate of 1.2 mL·min-1 ,the detector was set at 265 nm. Results: Linear range of NTF and KCZ was 0.08040 -2.010μg·mL-1 (r=0.9936) and 0. 2016-5.040μg·mL-1(r=0.9999) respectively,the detection limit was 0.0201μg·mL-1和0.0403μg·mL-1 respectively, the average recovery rate(n=15) was 102. 6% and 100. 1% respectively. Conclusion:This method is sensitive, accurate and reliable, and it is suitable for analysis of NTF and KCZ in vitro percutaneous solution, and can also be used for pharmacokinetic study.

关 键 词：HPLC法 离体透皮实验 萘替芬 酮康唑 药物浓度 NTF KCZ 

分 类 号：R446[医药卫生—诊断学]