丙型肝炎病毒核酸单引物聚合酶链反应产物的直接序列分析  

Direct sequencing of the product of single primer polymerase chain reaction of hepatitis C virus cDNA

作　　者：周荣[1] 孟庆华[1] 梁炽森[1] 骆抗先[1] 

机构地区：[1]南方医院传染病科

出　　处：《第一军医大学学报》1993年第4期334-335,共2页Journal of First Military Medical University

摘　　要：利用反转录聚合酶链反应(RT-PCR)技术,得到丙型肝炎病毒核酸非编码区引物所引导的扩增片段。用改进的不对称PCR方法,制备单链cDNA,直接作为模板进行序列分析,结果与Takamizawa发表的序列符合。本法简单方便,亦可用于其他类似的研究中。cDNA fragment was obtained by Reverse tianscription polymerase chain reaction (RT-PCR) using hepatitis C virus (HCV) non-coding region primers. Then this fragment was used as templet for asymmetric PCR to produce single strand (ss) HCV cDNA. This sscDNA can be used as templet for sequencing directly. The sequencing result is in complete accord with that in the report of Takamizawa. This method is simple and convenient, it can also be used in other molecular virology studies.

关 键 词：聚合酶链反应 核酸 丙型肝炎病毒 

分 类 号：R512.630.4[医药卫生—内科学]