噪声作业工人血浆热休克蛋白60、70抗体滴度分析  

An analysis on titer of antibody against heat shock protein 60 and 70 in the workers exposed to noise

作　　者：李毅[1] 姚惠琳[2] 祁成[2] 杨杪[3] 陈胜[3] 郑建如[2] 杨秋玲[2] 邬堂春[3] 

机构地区：[1]郧阳医学院附属东风医院检验部,湖北省十堰市442008 [2]东风汽车公司职业病防治所 [3]华中科技大学同济医学院公共卫生学院

出　　处：《中国全科医学》2004年第11期784-786,共3页Chinese General Practice

基　　金：国家自然科学基金项目 [编号 :30 2 0 0 2 2 7];本课题为湖北省科技攻关计划项目 [编号 :2 0 0 3AA30 1C1 0 7]

摘　　要：目的　探讨噪声作业工人血浆热休克蛋白 (HSPs) 6 0、 70抗体滴度及其与累积噪声暴露量 (CNE)的关系。方法　用Westernblot免疫印迹法对 391名工人 (噪声暴露 2 0 7名 ,对照 184名 )的血浆HSP 6 0、 70抗体水平进行测定 ,用趋势 χ2 检验和多因素Logistic回归模型分析HSP 6 0、 70抗体水平与噪声暴露的关系。结果　除HSP 6 0抗体滴度 1∶80外 ,其他滴度观察组HSP 6 0、 70抗体阳性率与对照组间差别均有显著性意义 ;HSP 70抗体滴度为 1∶10阳性率随累积噪声暴露量 (CNE)增大而升高 ;用Logistic回归分析控制了混杂因素的作用后 ,血浆HSP 6 0、HSP 70抗体检出率与CNE呈正相关关系。结论　血浆HSP 6 0、Objective To evaluate the titer of plasma antibody against heat shock protein (HSPs) in the workers exposed to noise,and the relationship between HSPs and cumulative noise expose (CNE).Methods Plasma anti-HSP 60 and anti-HSP 70 was determined with Western blot technique on 391 workers (207 cases for noise expose group,184 cases for control group).Trend Chi-square and Logistic regression model were used to analyze the relationship between the level of anti-HSPs and CNE.Results The positive rate of anti-HSP 70 (1∶10,1∶20,1∶40,1∶80) and anti-HSP 60(1∶10,1∶20,1∶40) in exposure group was significantly higher than that in control group.There was an increasing tendency of the positive rate of anti-HSP 70 (1∶10) along with the increasing of accumulative noise.After adjusted by the generally recognized risk factors,it still had a strong association between anti-HSPs and CNE.Conclusion The titer of plasma anti-HSP 60and anti-HSP 70 had close relationship with the worker′s cumulative noise expose (CNE).

关 键 词：噪声作业工人 血浆热休克蛋白60 70抗体滴度 累积噪声暴露量 

分 类 号：TB53[理学—物理]