阿司匹林诱导人肺腺癌细胞株SPCA-1凋亡研究  

Study of aspirin on proliferation and apoptosis of human lung Adenocarcinoma cell lines SPCA-1 in vivo

作　　者：朱慧明[1] 吴铁[1] 崔燎[2] 

机构地区：[1]广东医学院药理学教研室,湛江524023 [2]广东医学院广东天然药物研究与开发重点实验室,湛江524023

出　　处：《中国药理学通报》2004年第6期640-643,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助课题 ;No 3 970 90 0

摘　　要：目的　研究非甾体类抗炎药阿司匹林对肺腺癌SP CA 1体外增殖抑制作用及凋亡的影响。方法　用噻唑蓝(MTT)法测定细胞存活率 ;用流式细胞术测定细胞周期时相分布 ,瑞氏染色、Hoechest/PI双染、扫描电镜观察细胞表面形态改变 ,琼脂糖电泳法观察细胞凋亡。结果　阿司匹林对肺腺癌SPCA 1细胞有较强的抑制作用 ,有明显的时间和剂量依赖性 (1 0～ 12 5mmol·L-1) ;可使SPCA 1细胞G0 /G1期、G2 /M期比例明显升高 ,S期比例下降 ;凋亡细胞增多。结论　阿司匹林可能通过影响细胞周期时相分布、诱导凋亡以及引起细胞膜形态而抑制肺腺癌SPCA 1细胞增殖。AIM To study the effects of ASA on proliferation and apoptosis of human lung Adenocarcinoma cell lines SPCA-1 in vivo. METHODS Cytotoxicity assay was tested by MTT method.Cell cycle was analyzed by flow cytometry(FCM).The morphology of the treated cells was observed by wright exclusion,Hoechest/PI exclusion, electron microscope. Apoptosis landder was evaluated by agarose gel electrophoresis of DNA. RESULTS ASA inhibited SPCA-1 cell proliferation in a time-and dose-dependent fashion(1.0～12.5 mmol·L -1,24 h～72 h). ASA increased the number of cells in G 0/G 1 and G 2/M phases,degrased the population of S phases at on 24 h and incresed apoptosis cells number. CONCLUSION ASA may inhibit the proliferation of SPCA-1 cell lines through effects on cell cycle and apoptosis.

关 键 词：阿司匹林 非甾体类抗炎药 细胞毒作用 细胞周期 细胞凋亡 肺腺癌 SPCA-1 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]