东莨菪碱对吗啡依赖大鼠脑多巴胺转运蛋白及D_2受体的影响  

Effect of scopolamine on central DAT and D_2 receptor in morphine dependent rats

作　　者：林岩松[1] 丁时禹[2] 陈正平[2] 周翔[2] 方平[2] 王博诚[2] 王世真[1] 

机构地区：[1]中国医学科学院,中国协和医科大学北京协和医院核医学科,100730 [2]核医学国家重点实验室,江苏省原子医学研究所

出　　处：《中华核医学杂志》2004年第3期152-154,共3页Chinese Journal of Nuclear Medicine

基　　金：国家自然科学基金资助项目 (30 1 0 0 0 4 4 )

摘　　要：目的　探讨东莨菪碱 (Sco)对吗啡 (Mor)依赖大鼠多巴胺 (DA)系统多巴胺转运蛋白(DAT)及D2 受体的影响。方法　 4～ 5月龄SD大鼠 36只 ,随机分为Mor(按体重 2 0mg kg)组、Mor(按体重 2 0mg kg) +Sco组及生理盐水对照组。Mor+Sco组腹腔注射Mor前 15～ 30min先腹腔注射Sco(按体重 1mg kg) ,共给药 8d ;对照组只给予 0 3ml生理盐水。采用两隔室 (C1及C2 )地点偏爱装置测试大鼠的行为学变化。 3组大鼠再各随机均分为 2组 ,分别进行1 2 5I 甲基 3β (4 碘苯基 )托烷 2 β 羧酸酯 (β CIT)、1 2 5I 左旋 3 碘 2 羟基 6 甲氧基 N[(1 乙基 2 吡咯烷 )甲基 ]苯酰胺 (IBZM)脑内分布研究。结果　①Mor组大鼠第 2～ 4天起进入C2室的时间逐渐缩短 ,由第 1天的 (1 6 8± 0 5 7)min降至第 8天的 (0 38± 0 16 )min ;Mor+Sco组大鼠自C1进入C2室的时间第 1天至第 8天略有降低趋势 ,由第 1天的 (1 72± 0 6 9)min降至第 8天的 (1 12± 0 33)min ,第 1天 3组间差异无显著性 (P >0 0 5 ) ,第 8天Mor组及Mor+Sco组均低于对照组 [第 1天 :(1 6 0± 0 5 5 )min ,第 8天 :(1 88± 0 5 4 )min ;t=5 6 82、6 372 ,P均 <0 0 5 ],但Mor+Sco组仍高于Mor组 (t=5 171,P <0 0 5 )。②DAT分布研究示 :Mor组大鼠纹?Objective To investigate the effect of scopolamine (Sco) on central dopamine transporter (DAT) and D_2 receptor in morphine (Mor) dependent rats. Methods Chronic Mor exposure was induced by repeated Mor (20 mg·kg -1 ·d -1 , i.p.) treatment for 8 d. Conditioned place preference test was used to evaluate the drug seeking behavior. Biodistribution of the imaging agents 125 I-2β-carbomethoxy-3β-(4-iodophenyl)tropane (β-CIT) and 125 I-3-iodo-2-hydroxy-6-methoxy-N-[(1-ethoyl-2-pyrrolidinyl)methyl] benzamide (IBZM) were used to evaluate the central DAT and D_2 receptor during chronic Mor exposure. Results For the Mor plus pretreating with Sco (Mor+Sco) rats, the time for the rats entering C2 from C1 was (1.72±0.69) min in the first day, with little difference from the control and Mor group (P>0.05), and (1.12±0.33) min for the 8th day, still longer than that of the Mor group (t=5.171, P<0.05), but shorter than that of the control group (t=6.372, P<0.05), suggesting that Sco could prevent the trend of drug reinforcement induced by Mor to some extent. The 125 I-β-CIT %ID/g in striatum (ST) and nucleus accumbens (NAC) for Mor+Sco group were 3.307±0.189 and 1.577±0.401 respectively, higher than those of the control group (2.431±0.104, 1.441±0.043, t was 4.151 and 5.416 respectively, P<0.05), but lower than those of the Mor group rats (4.205±0.410, 1.770±0.141, t was 4.871 and 6.922 respectively, P<0.05). The 125 I-IBZM %ID/g in ST, NAC, hippocampus (HIP) and frontal cortex (FC) for Mor+Sco group were 0.589±0.081, 0.683±0.046, 0.175±0.039 and 0.257±0.034 lower than that of the control rats(0.735±0.096, 0.709±0.098, 0.281±0.038, 0.289±0.020, t was 7.841, 6.170, 5.446 and 4.337 respectively, P<0.05), but higher than those of the Mor group (0.540±0.098, 0.640±0.061, 0.140±0.017, 0.232±0.015, t was 6.021, 3.227, 5.113 and 6.174 respectively, P<0.05). Conclusions Sco could prevent the conditioned place preference of the rat induced by Mor, and it was mainly interaction with dopamine

关 键 词：东莨菪碱 吗啡依赖 脑 多巴胺 转运蛋白 D2受体 大鼠 

分 类 号：R96[医药卫生—药理学]